Ventana Medical Systems, Inc.

Cornell University

Ventana Medic Systems, Inc.

Other

Amo Development, LLC

Clmp, Inc.

Roche Diagnostics Gmbh

Quest Diagnostics Investments, Inc.

F. Hoffmann-la Roche Ag

Michael Otter

David Chafin

Daniel Bauer

Benjamin C Stevens

Karl Eric Garsha

Jefferson Taft

Nathan Crum

Brian Howard Kram

Gary A Pestano

Kevin David Marshall

Taylor J Shingler

Carl David Martin

Jerome W Kosmeder, Ii

Phillip C Miller

Eric J May

Esteban Roberts

Ray B Nagle

Mark A Rubin

Francesca Demichelis

Christine Tse

Josh Harrison

Ronald T Kurnik

William Kenneth Day

Wolfgang Petrich

Michael A Karavitis

William A Stark

Ronald Norman Diamond

Ruben Zadoyan

Peter Goldstein

Stacey Stanislaw

Chol Steven Yun

Timothy James Keller

Pascal Bamford

Jan Froehlich

Joshua David Kenneth Harrison

Evan T Graves

Niels Kroeger-Lui

Heidi Barnett

Abbey Pierson

Jessica Jones

Abbey Theiss

Joseph M Jakubowski

Alexandra Dea Nagy

Jessica A Wifall

Christian Roessler

James E Phillips-Portillo

Samuel Psota

Abbey Pierson Theiss

Kenneth S Weir

Benjamin Stevens

Ryan Dittamore

Frank Ventura

Karl Garsha

Alastair Laing

Thomas Sheng-Shyong Hu

Vinnie Petre

Mike White

Elizabeth Pigman

Patrick Brown

Ronald N Diamond

Marvin Arthur Petre

Ii Jerome W Kosmeder

Matthew D Mette

Jr John D Willems

Taylor Shingler

Timothy Keller

Jefferson Curtis Taft

Stanislaw Stacey

Daniel R Kurycki

Gary Anthony Pestano

A tissue sample that has been removed from a subject can be evaluated. A change in speed of the energy traveling through the sample is evaluated to monitor changes in the biological sample during processing. The monitoring can detect movement of fluid with the sample and cross-linking. A system for performing the method can include a transmitter that outputs the energy and a receiver configured to detect the transmitted energy.

Systems and methods for monitoring tissue sample processing

The subject disclosure presents systems and computer-implemented methods for evaluating a tissue sample that has been removed from a subject. A change in speed of the energy traveling through the sample is evaluated to monitor changes in the biological sample during processing. The rate of change in the speed of the energy is correlated with the extent of diffusion. A system for performing the method can include a transmitter that outputs the energy and a receiver configured to detect the transmitted energy. A time-of-flight of acoustic waves and rate of change thereof is monitored to determine an optimal time for soaking the tissue sample in a fixative.

Diffusion monitoring protocol for optimized tissue fixation

Disclosed are a system and method for evaluating a tissue sample that has been removed from a subject. Movement of fluid through the tissue sample is monitored by measuring time of flight of acoustic waves passed through the tissue sample. A system for performing the method can include a transmitter that outputs the energy and a receiver configured to detect the transmitted energy. Using the disclosed method and system, an optimized protocol for ensuring adequate distribution of the fluid throughout a variety of tissue types and/or sample sizes can be developed and utilized.

Standardizing diffusion of a fluid into tissue

Automated systems to make target compounds from slide stainer waste streams inactive utilizing advanced oxidation processes are described herein. Advanced oxidation processes are promoted by UV irradiation and further accelerated by use of radical initiators, such as hydrogen peroxide. The automated systems further include mechanisms for segregating components of the waste streams.

Passive, gravity-driven system for treatment of an effluent in a diagnostic system

A method for fixing a biological sample includes delivering energy through a biological sample that has been removed from a subject, while fixing the biological sample. A change in speed of the energy traveling through the biological sample is evaluated to monitor the progress of the fixation. A system for performing the method can include a transmitter that outputs the energy and a receiver configured to detect the transmitted energy. A computing device can evaluate the speed of the energy based on signals from the receiver.

Processing system for processing specimens using acoustic energy

A method is disclosed that permits calculation of reagent concentrations (in SI units) over time and space within a tissue sample as the sample is immersed in the reagent and the reagent diffuses into the tissue sample. The disclosed method has yielded the surprising result that once a formaldehyde concentration at all points within a tissue sample exceeds about 90 mM during a cold step of a cold+hot fixation protocol, the hot step of the fixation protocol can be commenced to provide reliable detection of molecular targets and preservation of tissue morphology in downstream analyses.

System and method for sample preservation

An aldehyde fixative solution at a first temperature is caused to contact a tissue sample for a first time period, additionally an aldehyde fixative solution is caused to contact the tissue sample at a second temperature higher than the first temperature for a second time period. The first time period typically ranges from about 15 minutes up to about 4 hours, and the first temperature typically is from greater than 0° C. to at least 15° C. The second temperature typically is from greater than about 22° C. to about 55° C., and the second time period ranges from about 1 hour to about 4 hours. Using this process, improved tissue morphology and IHC staining as well as superior preservation of post-translation modification signals have been accomplished in approximately 4 hours compared to 24 hours for room temperature protocols, and more even morphology and antigen preservation are observed.

Method for tissue sample fixation

A method and system are described for processing tissues according to particular processing protocols that are established based on time-of-flight measurements as a processing fluid is diffused into a tissue sample. In one embodiment, measurement of the time it takes about 70% ethanol to diffuse into a tissue sample is used to predict the time it will take to diffuse other processing fluids into the same or similar tissue samples. Advantageously, the disclosed method and system can reduce overall processing times and help ensure that only samples that require similar processing conditions are batched together.

System and method for sample processing

A method and system for processing a sample in a fluid is provided. An assembly comprising a cap prefilled with a fixative solution, a valve, and a container for storing a tissue sample are provided. The valve is adapted to be situated between the cap and the container such that fluid can flow from the cap into the container when the assembly is upright, but the fluid cannot backflow from the container to the cap when the assembly is horizontal or inverted.

Assembly for storing and transporting tissue samples immersed in a fluid

The subject disclosure presents systems and computer-implemented methods for determining an acoustic time-of-flight (TOF) of sound waves through a sample material with greater accuracy and in a more repeatable fashion, by invoking one or more of an envelope generation for an error function, fitting a non-linear curve to an ultrasound frequency sweep, or performing a clustered piece-wise linear regression on individual linear parts of the ultrasonic frequency sweep. The systems and methods are useful for, among other things, monitoring diffusion of fluids through porous materials, such as tissue samples.

Accurately calculating acoustic time-of-flight

Growing community of inventors

Tucson, AZ, United States of America

Michael Otter