Boehringer Mannheim Gmbh

Michael Nelbock-Hochstetter

Dieter Jaworek

Klaus Beaucamp

Hans Ulrich Bergmeyer

Karl-Heinz Botsch

Hans-Georg Batz

Wolfgang Gruber

Winfried Albert

Gunter Weimann

Gunter Lang

Helmut Determann

Jurgen Horn

Joseph Maier

Gunter Holz

Johanna Gramsall

Biologically active protein compositions comprising protein or a protein substrate bound onto a polyamide of the general formula: ##STR1## in which R and R.sub.1, which may be the same or different, are polyamide residues bound onto the amido group,

Polyamide-fixed biologically active protein

A new enzyme, cholesteroloxidase, is prepared by recovering same from microorganisms, preferably by washing a cholesterol-converting microorganism with butanol/water, disintegrating the microorganism culture and extracting same with a buffer solution containing a non-ionic surface-active agent, and isolating cholesterol oxidase from the extract.

Cholesteroloxidase and method for its production from microorganisms

Carrier-bound macromolecular compounds, especially bioactive macromolecular compounds, are produced by reacting a macromolecular compound A with a coupling compound B containing at least one reactive function capable of coupling with compound A and at least one further reactive function capable of polymerization, adding to the resulting coupled reaction product AB an unswollen molecular sieve material having cross-linking sufficient to exclude penetration by compound A, and polymerizing the polymerizable group of the coupled product AB in the molecular sieve material to form a polymer body in the molecular sieve material and fix compound A on the surface of the molecular sieve material. Compound A can be, e.g., an enzyme, antibody or hormone, and coupling compound B typically contains, as the coupable function, an alkylation or acylation group such as oxirane, ethyleneimine, halide, acid halide, azide or anhydride groups, and, as the polymerizable function, a carbon-carbon or carbon-oxygen double bond.

Preparation of carrier-bound macromolecular compounds

Biologically active proteins are bound onto an insoluble carrier by reacting such protein in an aqueous phase with a carrier comprising an activated polysaccharide having a hydrophilic graft co-polmyer grafted thereinto.

Process for binding biologically active proteins

Carrier-bound proteins are prepared by reacting a protein in aqueous solution with a coupling compound having at least one group capable of acylating or alkylating proteins and at least one additional group capable of producing a bond with a carrier material, and reacting the resulting product with a carrier material, optionally forming the carrier material in situ by polymerization of a monomer or monomer mixture in the presence of said product.

Carrier-bound protein prepared by reacting the protein with an acylating

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Tutzing, Germany

Michael Nelbock-Hochstetter