Sibia Neurosciences, Inc.

Merck + Co., Inc.

The Salk Institute Biotechnology Industrial Associates, Inc.

Phillips Petroleum Company

Other

University of Iowa Research Foundation

Research Corporation Technologies, Inc.

The Salk Institute Biotechnology/industrial Assoc, Inc.

Sibia Neuroscience, Inc.

Michael Miller Harpold

Steven B Ellis

Mark E Williams

Robert Brenner

Kathryn J Elliott

Ann F McCue

Daniel H Feldman

Paul Brust

Gonul Velicelebi

Michael Anthony Akong

David W Stroman

Arnold Schwartz

Juerg F Tschopp

Kevin P Campbell

James M Cregg

Alison Gillespie

Thomas R Gingeras

Paul F Brust

Scott David Jay

Jean Sartor

James Michael Cregg

Kenneth Stauderman

Frank H Gaertner

George T Sperl

Mary J Hagenson

Richard G Buckholz

Ronald D Klein

Kathryn A Barr

Juerg Friedrich Tschopp

David Womack Stroman

Mary Jane Hagenson

Isolated DNA encoding each of human calcium channel &#x3b1;, &#x3b1;, &#x3b2;- and &#x3b3;-subunits, including subunits that arise as splice variants of primary transcripts, is provided. Cells and vectors containing the DNA and methods for identifying compounds that modulate the activity of human calcium channels are also provided.

Human calcium channel compositions and methods

DNA encoding human neuronal nicotinic acetylcholine receptor alpha and beta subunits, mammalian and amphibian cells containing said DNA, methods for producing alpha and beta subunits and recombinant (i.e., isolated or substantially pure) alpha subunits (specifically &#x3b1;) and beta subunits (specifically &#x3b2;) are provided. In addition, combinations of a plurality of subunits (i.e., one or more of &#x3b1;, &#x3b1;, &#x3b1;, &#x3b1;, &#x3b1;, &#x3b1;and/or &#x3b1;subunits in combination with one or more of &#x3b2;subunits are provided.

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Nucleic acid molecules encoding human neuronal nicotinic acetylcholine receptor alpha and beta subunits, mammalian and amphibian cells containing the nucleic acid molecules, and methods for producing alpha and beta subunits are provided. In particular, nucleic acid molecules encoding &#x3b1;subunits and molecules encoding &#x3b2;subunits of human neuronal nicotinic acetylcholine receptors are provided. In addition, combinations of a plurality of subunits, such as one or more of &#x3b1;, &#x3b1;, &#x3b1;, &#x3b1;, &#x3b1;, &#x3b1;and/or &#x3b1;subunits in combination with one or more of &#x3b2;subunits or such as one or more of &#x3b2;, &#x3b2;and/or &#x3b2;subunits in combination with an &#x3b1;subunit are provided.

Human neuronal nicotinic acetylcholine receptor compositions and methods employing same

Isolated DNA encoding each of human calcium channel &#x3b1;-, &#x3b1;-, &#x3b2;- and &#x3b3;-subunits, including subunits that arise as splice variants of primary transcripts, is provided. Cells and vectors containing the DNA and methods for identifying compounds that modulate the activity of human calcium channels are also provided.

Nucleic acids encoding human calcium channel and methods of use thereof

DNA encoding human neuronal nicotinic acetylcholine receptor alpha and beta subunits, mammalian and amphibian cells containing the DNA, methods for producing &agr; and &bgr; subunits and isolated or substantially pure &agr; , &agr; and &bgr; subunits are provided. In addition, cells that expresses these subunits singly or combination with other subunits of nicotinic acetylcholine receptors and methods using the cells are provided.

Human neuronal nicotinic acetylcholine receptor & subunit methods employing same

Isolated DNA encoding each of human calcium channel &agr; -, &agr; -, &bgr;- and &ggr;-subunits, including subunits that arise as splice variants of primary transcripts, is provided. Cells and vectors containing the DNA and methods for identifying compounds that modulate the activity of human calcium channels are also provided.

Isolated nucleic acid encoding calcium channel &agr; -subunits, including subunits encoded by nucleic acid that arises as splice variants of primary transcripts, is provided. Cells and vectors containing the nucleic acid and methods for identifying compounds that modulate the activity of calcium channels that contain &agr; -subunits are also provided.

Calcium channel compositions and methods

Nucleic acid molecules encoding human neuronal nicotinic acetylcholine receptor alpha and beta subunits, mammalian and amphibian cells containing the nucleic acid molecules, and methods for producing alpha and beta subunits are provided. In particular, nucleic acid molecules encoding &agr; subunits and molecules encoding &bgr; subunits of human neuronal nicotinic acetylcholine receptors are provided. In addition, combinations of a plurality of subunits, such as one or more of &agr; , &agr; , &agr; , &agr; , &agr; , &agr; and/or &agr; subunits in combination with one or more of &bgr; subunits or such as one or more of &bgr; , &bgr; and/or &bgr; subunits in combination with an &agr; subunit are provided.

DNA encoding human neuronal nicotinic acetylcholine receptor alpha and beta subunits, mammalian and amphibian cells containing said DNA, methods for producing alpha and beta subunits and recombinant (i.e., isolated or substantially pure) alpha subunits (specifically &agr; ) and beta subunits (specifically &bgr; ) are provided. In addition, combinations of a plurality of subunits (i.e., one or more of as &agr; , &agr; , &agr; , &agr; , &agr; , &agr; and/or &agr; subunits in combination with one or more of &bgr; subunits are provided.

Human neuronal nicotinic acetylcholine receptor &agr;6 and &bgr;3 nucleic acid

Methods for identifying compounds which modulate the activity of human neuronal nicotinic acetylcholine receptors are provided. Invention methods employ DNAs encoding alpha and beta subunits of human neuronal nicotinic acetylcholine receptors, and the polypeptides encoded thereby. Test cells useful for conducting invention assays include mammalian and amphibian cells containing said DNA. Various combinations of subunits (i.e., one or more &agr; , &agr; , &agr; , &agr; , &agr; and &agr; subunits in combination with &bgr; , &bgr; and/or &bgr; subunits) can be employed for the invention assays.

Methods for identifying agonists and antagonists for human neuronal nicotinic acetylcholine receptors

Automated measurement apparatus and methods for automated drug screening procedures are provided. The apparatus is capable of initiating and measuring rapid or transient events, such as cell receptor and/or ion channel activity. In particular, the apparatus is capable of aligning with predetermined positions, one or more samples contained in a multi-well container, initiating the reaction with reagent addition and measuring a resultant attribute for a period of time. The apparatus can substantially continuously measure and record data corresponding to the measured attribute before, during and after initiation of the reaction so that a time course of the rapid or transient event may be determined. After the reaction(s) are complete in one or more wells of the multi-well container, the apparatus can align one or more different wells to be assayed with the predetermined position and repeat the cycle until a predetermined number of wells are assayed. Automated drug screening methods employing the apparatus are also provided for screening for compounds which activate, inhibit or potentiate cellular ion channel or receptor activity. The assays include fluorescent indicator-based assays that use viable cells containing effective levels of a fluorescent indicator which is responsive to changes in ion concentration. Activation of the ion channels or receptors is initiated in the automated measurement apparatus by injection into one or more predetermined cell-containing wells of a reagent which is a known or putative activator of the ion channels or receptors of interest. The resulting activity of the ion channels or receptors, which causes changes in ion concentration in the cytoplasm, is determined by measurement of fluorescence intensity changes of the indicator in response to an excitation wavelength.

Automated analysis equipment and assay method for detecting cell surface protein and/or cytoplasmic receptor function using same

DNA encoding human neuronal nicotinic acetylcholine receptor alpha and beta subunits, mammalian and amphibian cells containing the DNA, methods for producing &agr; and &bgr; subunits and isolated or substantially pure &agr;,, &agr;,and &bgr;,subunits are provided. In addition, cells that expresses these subunits singly or combination with other subunits of nicotinic acetylcholine receptors and methods using the cells are provided.

Automated drug screening method and a method of studying receptor and ion-channel activity are provided.

Automated analysis equipment and assay method for detecting cell surface

Isolated DNA encoding each of human calcium channel .alpha..sub.1 -, .alpha..sub.2 -, .beta.- and .gamma.-subunits, including subunits that arise as splice variants of primary transcripts, is provided. Cells and vectors containing the DNA and methods for identifying compounds that modulate the activity of human calcium channels are also provided.

Growing community of inventors

El Cajon, CA, United States of America

Michael Miller Harpold