Sapphire Energy, Inc.

The Scripps Research Institute

Calysta, Inc.

Renew Biopharma, Inc.

Michael Mendez

Yan Poon

Bryan O'Neill

Stephen P Mayfield

Joshua A Silverman

Sol M Resnick

Craig A Behnke

Kari Mikkelson

Luan Thanh Nguyen

Renee M Saville

Sungwon Lee

Matthew Saunders

Kyle Botsch

Joseph Noel

Michael D Burkart

Christopher Yohn

April Brand

Jeremy Lanoiselee

Nicole A Heaps

Philip Lee

Karla Gonzalez

Amy Curran

Shawn Szyjka

Wendy Levine

Stephen Mayfield

Described herein are variant, novel cannabinoid synthases, nucleic acids encoding same, and various uses thereof.

Variant cannabinoid synthases and methods and uses thereof

The disclosure relates to the biosynthesis of cannabinoids and related prenylated phenolic compounds using recombinant enzymes. In particular, the disclosure provides recombinant mutant ORF2 enzymes engineered to produce a greater amount of a desired product, or to have a greater ability to catalyze a reaction using a desired substrate, as compared to WT ORF2. The disclosure also provides methods of preparing such ORF2 mutant enzymes; as well as methods of use thereof in improving the biosynthesis of cannabinoids and related prenylated phenolic compounds.

Compositions and methods for using genetically modified enzymes

The present disclosure relates to bioengineering approaches for producing biofuel and, in particular, to the use of a Cmetabolizing microorganism reactor system for converting Csubstrates, such as methane or methanol, into biomass and subsequently into biofuels, bioplastics, or the like.

Biorefinery system, methods and compositions thereof

The present disclosure provides novel proteins that when over expressed in algae result in an increase or change in fatty acid and/or glycerol lipid production and/or accumulation, without a substantial decrease in the growth rate of the alga or the break down of algal components, such as chlorophyll. The present disclosure also describes methods of using the novel proteins to increase or change the production and/or accumulation of fatty acids and/or glycerol lipids in algae. In addition, these proteins are useful tools in obtaining information about the fatty acid and triacyglyceride (TAG) synthetic pathways in algae.

Transformation of algae for increasing lipid production

This disclosure provides methods of designing and generating polypeptide variants that have altered properties compared to a parent polypeptide. The present disclosure provides methods of generating polypeptide variants, for example, variant isoprenoid synthases and/or variant prenyl transferases that have at least one desired property not present in the parent polypeptide. The present disclosure further provides polypeptides and polynucleotides encoding variant polypeptides, as well as vectors and host cells comprising the polynucleotides that encode the variant polypeptides. In other embodiments, the present disclosure provides methods of using the variant polypeptides to generate useful products, such as isoprenoid compounds and/or isoprenoid products.

Variant isoprenoid producing enzymes and uses thereof

The present invention provides compositions and methods for producing flocculation moieties in photosynthetic organisms. The photosynthetic organisms are genetically modified to effect production, secretion, or both, of the flocculation moieties. Also provided are methods of flocculating organisms.

Induction of flocculation in photosynthetic organisms

The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.

High throughput screening of genetically modified photosynthetic organisms

Use of genetically modified organisms to generate biomass degrading enzymes

The functional analysis of genes frequently requires the manipulation of large genomic regions. A yeast-bacteria shuttle vector is described that can be used to clone large regions of DNA by homologous recombination. Also described is a method for isolating entire genomes, including chloroplast genomes, or large portions thereof, and manipulating the same. Also described are methods for determining minimal genomes, minimal pathway requirements, and minimal organelle genomes.

System for capturing and modifying large pieces of genomic DNA and constructing organisms with chloroplasts

Growing community of inventors

San Diego, CA, United States of America

Michael Mendez