Institut National De La Sante Et De La Recherche Medicale

Other

Assistance Publique-Hopitaux De Paris

Université Paris Est Creteil Val De Marne

Etablissement Francais Du Sang

Meristern Therapeutics

Institut National De La Sante Et De La Recherche Medicale (Inserm)

Michael Marden

Veronique Gruber

Bertrand Merot

Philippe Lenee

Sylvie Baudino

Claude Poyart

Wilfrid Dieryck

Renee-Josee Pagnier

Pablo Bartolucci

France Pirenne

Laurent Kiger

The aim of the present invention is to propose a novel method for spectral study of a biological fluid from an absorption spectrum of a sample of the biological fluid. The inventors have made an original use of spectroscopy in order to accurately determine the protein content of a biological sample, in particular for oxyhaemoglobin, methaemoglobin, bilirubin and the haem bound to serum albumin. Such a method can be used in a variety of applications concerning haem- or haemoprotein- related diseases, in particular diagnostic methods, treatment follow-up methods, methods for determining biomarkers or screening methods. Such a method is also advantageous for qualifying blood bags.

Method for spectral study of a biological fluid

A method for producing haemin proteins by (i) inserting into plant cells one or more nucleic acid molecules that each comprise at least one sequence coding for a protein component of an animal haemin protein capable of reversibly binding oxygen, or for a variant or portion of said protein component, and optionally a sequence coding for a selection agent; (ii) selecting cells containing nucleic acid coding for the protein component of the haemin protein; (iii) optionally propagating the transformed cells either in a culture or by regenerating whole transgenic or chimeric plants; and (iv) recovering and optionally purifying a haemin protein that includes a complex consisting of the protein or proteins coded for by said nucleic acid and at least one iron-containing porphyritic nucleus, or a plurality of such complexes.

Method for producing hemin proteins using plant cells, resulting proteins and products containing same

A method for producing human hemoglobin proteins by (i) inserting into plant cells one or more nucleic acid molecules that each comprise at least one sequence coding for a protein component of a human hemoglobin protein capable of reversibly binding oxygen, and optionally a sequence coding for a selection agent; (ii) selecting cells containing nucleic acid coding for the protein component of the human hemoglobin protein; (iii) optionally propagating the transformed cells either in a culture or by regenerating whole transgenic or chimeric plants; and (iv) recovering and optionally purifying the human hemoglobin protein that includes a complex consisting of the protein or proteins coded for by the nucleic acid and at least one iron-containing polphyritic nucleus, or a plurality of such complexes.

Method for producing human hemoglobin proteins using plant cells

Growing community of inventors

Aulnay-sous-Bois, France

Michael Marden