Shenzhen Nanobioimaging Limited

Michael M T Loy

Shengwang Du

Teng Zhao

A two-color fluorescence localized super-resolution biological microscopy method and system are disclosed. The method includes: performing two-color fluorescence labeling on a biological sample by using Alexa647 and Alexa750 fluorescent molecules or Cy5 and Cy7 fluorescent molecules, and soaking the biological sample in an imaging buffer solution; illuminating the biological sample by laser to generate a flashing fluorescent signal of a first channel and a flashing fluorescent signal of a second channel respectively; constructing a super-resolution image of a first biological structure and a super-resolution image of a second biological structure respectively; and aligning the super-resolution image of the first biological structure and the super-resolution image of the second biological structure so as to construct a super-resolution image of a third biological structure. Thereby, the present method and system do not generate channel crosstalk and can significantly reduce background noise, so that the imaging quality is improved.

Growing community of inventors

Hong Kong, China

Michael M T Loy