Harvard College

Tufts College

Tufts University

University of Vermont

Michael Levin

Donald Elliot Ingber

David L Kaplan

Richard Novak

Josh Bongard

Sam Kriegman

Kelly A McLaughlin

Rachelle Prantil-Baun

Douglas J Blackiston

Nirosha J Murugan

Disclosed are engineered multicellular organisms. The disclosed organisms comprise an aggregate of ciliated cells, and the organisms move when the ciliated cells are actuated. The engineered multicellular organisms also are capable of kinematic self-replication. The engineered multicellular organisms are capable of moving a plurality of dissociated ciliated cells into piles of ciliated cells which form a multicellular organism comprising an aggregate of ciliated cells which moves when the ciliated cells are actuated. Also disclosed are systems and methods for designing, preparing, and utilizing the engineered multicellular organisms.

Engineered multicellular ciliated organisms and kinematic self-replication thereof

Described herein are microfluidic devices and systems for high density cell culture and/or high throughput cell assays. Methods of using the same are also provided herein. In some embodiments, the microfluidic devices and systems described herein provide rapid and automated trapping of single embryos in ordered arrays.

Microfluidic devices and systems for cell culture and/or assay

Growing community of inventors

Beverly, MA, United States of America

Michael Levin