Bio-Rad Laboratories, Inc.

Cholestech Corporation

Michael Leos

Renato B Del Rosario

William F Link

David L King

Anthony Joseph Nugent

Thomas E Worthy

Jeffrey Shindelman

Neal F Bellet

Kimberly Haley

Leanne M Cowley

David King

The present disclosure provides compositions and methods that are useful for normalizing the amount of signal detected in an assay, such as an immunoassay. The compositions and methods are useful for improving the accuracy of immunoassays, such as immunoassays that detect whether a subject is infected with a retrovirus such as HIV.

Human factor XIII as a normalization control for immunoassays

An immunoassay cassette, apparatus, and method for detecting an analyte in a liquid body-fluid sample are disclosed. The cassette has a body and a support mounted on the body, for movement toward and away from a sample transfer position. Sample supplied to a sample well in the cassette body is taken up by a reagent reservoir containing a first reagent composition effective to form a modified sample. The support provides a reagent strip having a transfer zone that is brought into contact with the reservoir, when the support is in the transfer position, a detection zone located downstream of the transfer zone, and a second reagent composition effective to react with the modified sample to form a detectable analyte-dependent product. By controlling movement of the support, volume and rate of sample flow from the reservoir to the strip can be controlled to optimize and/or standardize sample-transfer conditions in the assay.

Automated immunoassay cassette, apparatus and method

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Fairfield, CA, United States of America

Michael Leos