Syngenta Participations Ag

University of California

Syngenta Crop Protection Ag

Rhobio

Bayer Cropscience S.a.

Michael L Nuccio

Brent Delzer

Robert Arthur Dietrich

Timothy Kelliher

Todd Lee Warner

William Paul Bullock

Suresh Babu Kadaru

Satya P Chintamanani

Mikyong Lee

Joseph Michael Clarke

Moez Rajabali Meghji

L Mark Lagrimini

Georges L Freyssinet

Jonathan Cohn

Avutu S Reddy

Terry L Thomas

Shib Sankar Basu

Sean R Cutler

Weining Gu

Joseph Dallas Clarke

John Steffens

Tzung-Fu Hsieh

Kateri Duncan

Lawrence Mark Lagrimini

Satya Chintamanani

Royston E Carter

A Todd Richmond

Quideng Que

Andrew N Nunberg

Mark L Lagrimini

Provided here are methods of using a mutated patatin-like phospholipase IIα ('pPLAIIα,' renamed here MATRILINEAL) to induce haploid induction in plants, cloning a pPLAIIα to induce haploid induction in plants, and genetically engineering a plant to contain a mutated pPLAIIα. Also provided are methods of applying topical and spray chemicals, lipids, and RNAi molecules to plants during pollination in order to induce haploid production. Further provided are methods of chemically treating plants during pollination to induce haploids while also reducing embryo abortion and increasing seed set.

Haploid induction compositions and methods for use therefor

Provided are isolated cDNAs comprising a nucleotide sequence having at least 90% identity to SEQ ID NO: 33, SEQ ID NO: 37, SEQ ID NO: 52 or SEQ ID NO: 53. Also provided are expression cassettes; vectors; transgenic plant cells; plants, plant parts, and seeds; isolated polypeptides; amplicons and informative fragments of the presently disclosed nucleic acids; compositions that include amplification primer pairs; methods for producing plants that exhibit HI; methods for identifying the presence or absence of an allele associated with HI in a plant; methods for introgressing Haploid-inducing nucleotide sequences into plants; and methods for selecting parental plants predicted to produce progeny generations with plants that exhibit Haploid Induction trait.

Provided here are methods of using a mutated patatin-like phospholipase II&#x3b1; ('pPLAII&#x3b1;,' renamed here MATRILINEAL) to induce haploid induction in plants, cloning a pPLAII&#x3b1; to induce haploid induction in plants, and genetically engineering a plant to contain a mutated pPLAII&#x3b1;. Also provided are methods of applying topical and spray chemicals, lipids, and RNAi molecules to plants during pollination in order to induce haploid production. Further provided are methods of chemically treating plants during pollination to induce haploids while also reducing embryo abortion and increasing seed set.

The present disclosure relates to regulatory sequences. In particular, the disclosure relates to a regulatory nucleic acid molecule, at least part of which has a transcription initiation function directing expression of an operably associated protein encoding polynucleotide of interest to non-tassel tissue in maize, but not or substantially not to tassel. The disclosure further relates to chimeric genes and expression cassettes comprising the regulatory nucleic acid molecule and to transgenic plants comprising the chimeric genes and expression cassettes.

Plant regulatory sequence

Provided are isolated cDNAs comprising a nucleotide sequence having at least 90% identity to SEQ ID NO: 33, SEQ ID NO: 37, SEQ ID NO: 52 or SEQ ID NO: 53. Also provided are expression cassettes; vectors; transgenic plant cells; plants, plant parts, and seeds; isolated polypeptides; amplicons and informative fragments of the presently disclosed nucleic acids; compositions that include amplification primer pairs; methods for producing plants that exhibit HI; methods for identifying the presence or absence of an allele associated with HI in a plant; methods for introgressing Haploid&#x2014;inducing nucleotide sequences into plants; and methods for selecting parental plants predicted to produce progeny generations with plants that exhibit Haploid Induction trait.

Hypersensitive PYR/PYL polypeptides, compositions, and methods are provided.

Hypersensitive ABA receptors

The present invention relates to regulatory sequences. In particular, the invention relates to a regulatory nucleic acid molecule, at least part of which has a transcription initiation function directing expression of an operably associated protein encoding polynucleotide of interest to non-tassel tissue in maize, but not or substantially not to tassel. The invention further relates to chimeric genes and expression cassettes comprising the regulatory nucleic acid molecule and to transgenic plants comprising the chimeric genes and expression cassettes.

In one aspect, the present invention provides an expression cassette comprising a promoter operably linked to a nucleic acid molecule of interest, which is operably linked to a terminator, further comprising a heterologous intron that enhances expression of the nucleic acid molecule of interest, wherein the heterologous intron is selected from the group comprised of SEQUENCE ID NO. 1 (iZm10430-02), SEQUENCE ID NO. 2 (iZm10430-01) SEQUENCE ID NO. 3 (iZm008975-01), SEQUENCE ID NO. 4 (iZm005854-01), SEQUENCE ID NO. 5 (iZm010719-01), and SEQUENCE ID NO. 6 (iZm007840-01). In another aspect, the present invention provides an expression cassette comprising a promoter operably linked to a nucleic acid molecule of interest, which is operably linked to a heterologous terminator that enhances expression of the nucleic acid molecule of interest, wherein the heterologous terminator is selected from the group of SEQUENCE ID NO. 7 (tZmHSP70-01), SEQUENCE ID NO. 8 (tZmUbi158-01), SEQUENCE ID NO. 9 (tZmUbi1-01), SEQUENCE ID NO. 10 (tZmUbi361-01).

Methods and compositions for expression cassettes comprising a maize gene-derived intron for enhanced expression

The present invention includes expression cassettes that contain regulatory sequences derived from a target gene, for example, regulatory sequences from the HSP70, Ubi158, and Ubi361 genes, for expression of recombinant gene products in plants. Developmental expression profiling data were used to identify several gene candidates for strong constitutive expression cassette development. Three expression cassettes were developed. They are based on the ZmHSP70, ZmUbi158, and ZmUBI361 genes.

Expression cassettes derived from maize

Provided are expression cassettes; vectors; transgenic plant cells; plants, plant parts, and seeds; isolated polypeptides; amplicons and informative fragments of the presently disclosed nucleic acids; compositions that include amplification primer pairs; methods for producing plants that exhibit haploid induction (HI); methods for identifying the presence or absence of an allele associated with HI in a plant; methods for introgressing Haploid-inducing nucleotide sequences into plants; and methods for selecting parental plants predicted to produce progeny generations with plants that exhibit Haploid Induction trait.

The present invention relates generally to the field of molecular biology and regards various polynucleotides, polypeptides and methods that may be employed to enhance yield in transgenic plants. Specifically the transgenic plants may exhibit any one of the traits consisting of increased yield, increased tolerance to abiotic stress, increased cell growth and increased nutrient use efficiency.

Growing community of inventors

Durham, NC, United States of America

Michael L Nuccio