Monsanto Company

Vysis, Inc.

Other

Amoco Corporation

Michael L Bittner

Larry E Morrison

Mona S Legator

Edith Wong

Heinz Hermann Busta

Mark J Quintile

Kenneth A Cruickshank

Richard E Cuellar

Marilyn S Mai

Sarah R Braford

Clarissa F Prorok

Dale Maynard

Lucy M Stols

A frame member fastening device and method of manufacture provides a single configuration fastening device which can be used singly in connection with a frame member, or which can be combined with another identical fastening device to form a fastening device assembly for use with frame members of different internal widths, by interlocking two identical fastening devices in an opposed, base-to-base assembly configuration. The fastening device is manufactured in a progressive metal forming process with successive stages in which the features of the device are formed.

Frame member fastening device and method of manufacture

Direct label probe compositions which stain DNA of a preselected single chromosome or region of a chromosome of a multi-chromosomal genome are provided that comprise mixed DNA segments which are covalently bound to fluorophore groups through linking groups. The mixed DNA segments are derived from the DNA present in the preselected chromosome or chromosome region. These probe compositions can be used concurrently or sequentially with other probe compositions.

Methods for multiple direct label probe detection of multiple chromosomes or regions thereof by in situ hybridization

A method for bacterially producing IGF-I is disclosed in which Gram-negative bacteria are caused to express a gene consisting of a lamB or ompF signal sequence operatively joined to a DNA sequence encoding IGF-I and producing IGF-I which is secreted into the periplasmic space of the bacteria.

Secretion of insulin-like growth factor-I in E. coli

Direct label probe compositions which counterstain genomic DNA are provided that comprise mixed DNA segments which are covalently bound to fluorophore groups through linking groups. The mixed DNA segments are derived from the total genomic DNA of a multi-chromosomal organism, especially man, and the segment mixture is approximately representative of such total genomic DNA. These probe compositions can be used concurrently or sequentially with other probe compositions. Instead of intercalating with DNA as in prior art chemical counterstains, these probe compositions hybridize with complementary segments that are present in the genomic DNA of a specimen.

Methods for genome identification using direct label probe composition

Methods for detection by in situ hybridization of multiple chromosomes

Probe compositions for chromosome identification and methods

DNA comprising lamb secretion signal sequence and mature IGF-1 sequence

Techniques for producing cloned DNA sequences are provided which sequences are complementary to DNA occurring in one selected region of one chromosome of a multi-chromosomal genome, such as the human genome. Such cloned DNA sequences can be labeled and formed into probes by conventional procedures, there are provided methods for making probe compositions which comprise mixed DNA segments derived from such a DNA sequence. An improved DNA sequence transamination procedure is provided utilizing trifluoroacetate chaotrope anions. With high concentrations of low complexity DNA, high levels of transamination are thereby achieved. These segments are covalently bound to fluorophore groups through linking groups that are transaminated preferably chaotropically into the segments.

Production of chromosome region specific DNA sequences and transamination

Direct label transaminated DNA probe compositions for chromosome

A method for bacterially producing IGF-I is disclosed in which Gram-negative bacteria are caused to express a gene consisting of a lamb or ompF signal sequence operatively joined to a DNA sequence encoding IGF-I and producing IGF-I which is secreted into the periplasmic space of the bacteria.

An electrode set for electrotransformation of a host cell by electroporation, comprising: an electrically insulating substrate; and at least one pair of interdigitated electrodes which are carried by said substrate and which lie in a generally planar array, said electrodes and said substrate being adopted to carry a cell transformant and a host cell thereon, said electrodes and said host cell and said cell transformant being adapted to pass electrical current upon the application of a potential difference across said electrodes.

Apparatus and method for electroporation

A method for obtaining heterologous peptides from fusion proteins wherein heterologous peptides include eucaryotic hormones such as atrial peptides. A novel DNA sequence encoding atrial peptide III. Various genes, DNA vectors, endopeptidases and transformed bacteria useful in practicing the method of the present invention.

Release of recombinant peptides from polypeptides using V8 endopeptidase

Secretion of insulin-like growth factor-I

This invention relates to the expression and accumulation in bacterial cells of heterologous polypeptides under the control of the recA promoter/operator region. One such polypeptide comprises a recA/somatostatin fusion polypeptide. This fusion polypeptide has been expressed and accumulated by E. coli with much greater effectiveness than other somatostatin-containing fusion polypeptides. In addition, use of the recA promoter/opeerator region provides greater induction flexibility than other inducible promoter systems.

Growing community of inventors

Naperville, IL, United States of America

Michael L Bittner