Lanzatech New Zealand Limited

Lanzatech, Inc.

Lanzatech Nz, Inc.

Northwestern University

The University of Queensland

Michael Koepke

Fungmin Liew

Sean Dennis Simpson

Shilpa Nagaraju

Alexander Paul Mueller

Rasmus Overgaard Jensen

James Bruce Yarnton Haycock Behrendorff

Wendy Yiting Chen

Simon David Oakley

Loan Phuong Tran

Christophe Collet

Stephanie Rhianon Jones

Esteban Marcellin

Michael Cockrem

Kaspar Valgepea

Bakir Al-Sinawi

Lars K Nielsen

Ryan Edward Hill

David Jeffrey Fraser Walker

James MacAllister Clomburg

Timothy James Politano

Juniper Dee Gramling

Jaeyoung Jung

Ryan Christopher Tappel

Michael Christopher Jewett

Robert John Conrado

Richard Llewellyn Sydney Forster

Michael Charles Milner Cockrem

Christophe Daniel Mihalcea

Allan Haiming Gao

Jason Carl Bromley

Guy William Waters

Simon Richard Trevethick

Wyatt Eric Allen

Bjorn Daniel Heijstra

Ching Leang

Lars Keld Nielsen

Rasmus Jensen Overgaard

Wayne Michael Patrick

Danielle Joan Maddock

Monica Gerth

Evgenia Kern

Wendy Chen

Simon Segovia

Sashini De Tissera

Steven Brown

James Daniel Winkler

Peter Dürre

Nicholas Alexander Fackler

Zachary Robert Cowden

Renato De Souza Pinto Lemgruber

Renato De Sp Lemgruber

James Daniell

Audrey Harris

Darmawi Juminaga

Antje Kruger-Gericke

Renato De S P Lemgruber

Renato De Lemgruber

Lars Nielsen

Renato De SP Lemgruber

James Bruce Behrendorff

David Jeffrey Walker

Microorganisms are genetically engineered to continuously co-produce amino acids, proteins, microbial biomass, chemicals, or any combination thereof by microbial fermentation, particularly by microbial fermentation of a gaseous substrate. The microorganisms are C1-fixing. The production of ethylene, microbial biomass, and heterologous tandem repeat proteins can be improved. This can be effected by improved promoters or nutrient limiting means.

Microorganisms and methods for the continuous co-production of tandem repeat proteins and chemical products from C1-substrates

Microorganisms are genetically engineered to continuously co-produce amino acids, high-value, specialized proteins, microbial biomass, chemicals, or any combination thereof by microbial fermentation, particularly by microbial fermentation of a gaseous substrate. The microorganisms are C1-fixing. The production of ethylene, microbial biomass, and heterologous high-value, specialized proteins can be improved. This can be improved by varying promoters or nutrient limiting means.

Microorganisms and methods for the continuous co-production of high-value, specialized proteins and chemical products from C1-substrates

The invention provides a method for producing a terpene or a precursor thereof by microbial fermentation. Typically, the method involves culturing a recombinant bacterium in the presence of a gaseous substrate whereby the bacterium produces a terpene or a precursor thereof, such as mevalonic acid, isopentenyl pyrophosphate, dimethylallyl pyrophosphate, isoprene, geranyl pyrophosphate, farnesyl pyrophosphate, and/or farnesene. The bacterium may comprise one or more exogenous enzymes, such as enzymes in mevalonate, DXS, or terpene biosynthesis pathways.

Microbial fermentation for the production of terpenes

The disclosure provides genetically engineered C1-fixing microorganisms capable of producing nanobodies. Additionally, the disclosure provides engineered microorganisms comprising one or more disrupted genes to strategically divert carbon flux away from nonessential or undesirable products towards products and/or co-products of interest. The disclosure enables co-production of useful chemicals from gaseous substrates.

Recombinant microorganisms and uses therefor

Disclosed herein are improved methods for production of 2-phenylethanol by microbial fermentation of substrates comprising carbon monoxide and/or carbon dioxide and further disclosed are genetically modified microorganisms for use in such methods that alleviate dependence on natural and petrochemical processes.

Microorganism for fermentative production of 2-phenylethanol from gaseous substrates

Disclosed are compositions, methods, and kits for performing cell-free RNA transcription and/or cell-free protein synthesis (CFPS). The disclosed compositions, methods, and kits include or utilize components prepared from a species of Clostridia such as cellular extracts from

Cell-free protein synthesis platforms derived from clostridia extracts

Provided herein are microorganisms and methods for fermentative production of β-ketoadipate from gaseous substrates such as carbon dioxide (CO), carbon monoxide (CO), and/or hydrogen (H). Additionally, the processes provided herein are methods for producing polymers containing β-ketoadipate, that can potentially enable a circular economy by diverting waste, e.g., plastic waste.

Fermentative production of B-ketoadipate from gaseous substrates

The invention provides methods for improving efficiency of fermentation by arginine supplementation, and genetically modified bacterium for use therefor. More particularly the invention provides methods for (i) increasing the production ATP intensive products with arginine supplementation, (ii) increasing utilization of arginine by a C1-fixing bacterium; and (iii) providing C1-fixing bacterium with optimized arginine de-aminase pathways.

Arginine supplementation to improve efficiency in gas fermenting acetogens

The disclosure provides genetically engineered microorganisms and methods for improved biological production of ethylene glycol and precursors of ethylene glycol. The microorganism of the disclosure produces ethylene glycol or a precursor of ethylene glycol through one or more of 5,10-methylenetetrahydrofolate, oxaloacetate, citrate, malate, and glycine. The disclosure further provides compositions comprising ethylene glycol or polymers of ethylene glycol such as polyethylene terephthalate.

Microorganisms and methods for improved biological production of ethylene glycol

Microorganisms are genetically engineered to produce 3-hydroxypropionate (3-HP). The microorganisms are carboxydotrophic acetogens. The microorganisms produce acetyl-coA using the Wood-Ljungdahl pathway for fixing CO/CO. A &#x3b2;-alanine pyruvate aminotransferase from a microorganism that contains such an enzyme is introduced. Additionally, an acetyl-coA carboxylase may also be introduced. The production of 3-HP can be improved. This can be effected by improved promoters or higher copy number or enzymes that are catalytically more efficient.

The disclosure relates to the combination of a primary fermentation that converts gas to acetate with a secondary fermentation that converts acetate to a target product. Preferably, the gas contains carbon dioxide, such that the disclosure enables the fixation of carbon dioxide into useful products. The fermentations may be any combination of aerobic and anaerobic, batch and continuous. The fermenting microorganisms may typically be bacterial or fungal.

Secondary acetate fermentation

The invention provides genetically engineered microorganisms and methods for the biological production of ethylene glycol and precursors of ethylene glycol. In particular, the microorganism of the invention produces ethylene glycol or a precursor of ethylene glycol through one or more of 5,10-methylenetetrahydrofolate, oxaloacetate, citrate, malate, and glycine. The invention further provides compositions comprising ethylene glycol or polymers of ethylene glycol such as polyethylene terephthalate.

Microorganisms and methods for the biological production of ethylene glycol

Provided is a method of producing a product by culturing a carboxydotrophic acetogenic bacterium with a disrupting mutation in a lactate dehydrogenase enzyme in the presence of a substrate comprising CO, CO, and/or H. Preferably, the disrupting mutation reduces or eliminates the expression or activity of the enzyme such that the bacterium produces a reduced amount of lactate or no lactate.

Recombinant microorganisms and methods of use thereof

The invention provides methods of genetically engineering a C1-fixing bacterium using a Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) (CRISPR/Cas) system. Preferably, the Cas protein is under the control of an inducible promoter.

CRISPR/cas systems for c1-fixing bacteria

Growing community of inventors

Skokie, IL, United States of America

Michael Koepke