Other

Erasmus Universiteit Rotterdam

Michael Kneba

Jacobus Johannes Maria Van Dongen

Christian Bastard

Anthonie Willem Langerak

Michael Hummel

Eduardus Maria Dominicus Schuuring

Antonio Parreira

John Lewis Smith

Frances Louise Lavender

Gareth John Morgan

Paul Anthony Stuart Evans

Elizabeth Anne Macintyre

Ramon Garcia Sanz

Jesus Fernando San Miquel

Jesus Fernando San Miguel

Frederic Bernard Louis Davi

Monika Brüggemann

Ramon Garzia Sanz

The invention relates to PCR-based clonality studies for among others early diagnosis of lymphoproliferative disorders. Provided is a set of nucleic acid amplification primers comprising a forward primer, or a variant thereof, and a reverse primer, or a variant thereof, capable of amplifying a rearrangement selected from the group consisting of a VH-JH IGH rearrangement, a DH-JH IGH rearrangement, a VK-J&#x3ba; IGK rearrangement, a VK/intron-Kde IGK rearrangement, a V&#x3bb;-J&#x3bb; IGL rearrangement, a V&#x3b2;-J&#x3b2; TCRB rearrangement, a D&#x3b2;-J&#x3b2; TCRB rearrangement, a V&#x3b3;-J&#x3b3; TCRG rearrangement, a V&#x3b4;-J&#x3b4; TCRD rearrangement, a D&#x3b4;-D&#x3b4; TCRD rearrangement, a D&#x3b4;-J&#x3b4; TCRD rearrangement, a V&#x3b4;-D&#x3b4; TCRD rearrangement, or a translocation selected from t(11;14)(BCL1-IGH) and t(14;18)(BCL2-IGH). The primers can be used in PCR-based clonality studies for early diagnosis of lymphoproliferative disorders and detection of minimal residual disease (MRD). Also provided is a kit comprising at least one set of primers of the invention.

Nucleic acid amplification primers for PCR-based clonality studies

The invention relates to PCR-based clonality studies for among others early diagnosis of lymphoproliferative disorders. Provided is a set of nucleic acid amplification primers comprising a forward primer, or a variant thereof, and a reverse primer, or a variant thereof, capable of amplifying a rearrangement selected from the group consisting of a VH-JH IGH rearrangement, a DH-JH IGH rearrangement, a VK-JK IGK rearrangement, a VK/intron-Kde IGK rearrangement, a V&#x3bb;-J&#x3bb; IGL rearrangement, a V&#x3b2;-J&#x3b2; TCRB rearrangement, a D&#x3b2;-J&#x3b2; TCRB rearrangement, a V&#x3b3;-J&#x3b3; TCRG rearrangement, a V&#x3b4;-J&#x3b4; TCRD rearrangement, a D&#x3b4;-D&#x3b4; TCRD rearrangement, a D&#x3b4;-J&#x3b4; TCRD rearrangement, a V&#x3b4;-D&#x3b4; TCRD rearrangement, or a translocation selected from t(11;14)(BCL1-IGH) and t(14;18)(BCL2-IGH). The primers can be used in PCR-based clonality studies for early diagnosis of lymphoproliferative disorders and detection of minimal residual disease (MRD). Also provided is a kit comprising at least one set of primers of the invention.

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Westensee, Germany

Michael Kneba