Promega Corporation

Michael Killoran

Lance P Encell

Mary P Hall

Thomas A Kirkland

Ce Shi

Poncho Meisenheimer

Keith V Wood

Melanie Dart

Trish Hoang

Virginia Kincaid

Provided herein are bioluminescent polypeptides and compositions and methods for the assembly of a tripartite or multipartite bioluminescent complex. In particular embodiments, a bioluminescent complex is formed upon the interaction of three or more peptide and/or polypeptide components.

Multipartite luciferase peptides and polypeptides

Provided herein are enhanced luciferase enzymes for use with thermostable luciferin analogs for bioluminescent assays. In particular, the present disclosure provides compositions, assays, and methods for performing a bioluminescent assay using enhanced, high-activity luciferase enzymes compatible with thermostable luciferins, such as 5,5-disubstituted luciferin analogs.

Luciferase enzymes for use with thermostable luciferins in bioluminescent assays

Described are luciferin analogues, methods for making the analogues, kits including the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays.

5,5-disubstituted luciferins and their use in luciferase-based assays

Described are luciferin analogs, methods for making the analogs, kits including the analogs, and methods of using the compounds for the detection of luminescence in luciferase-based assays.

Growing community of inventors

Fitchburg, WI, United States of America

Michael Killoran