E.i. Dupont De Nemours and Company

Michael K Hanafey

Scott Anthony Sebastian

Scott Valray Tingey

J Antoni Rafalski

Tina K Van Dyk

Robert A LaRossa

Gregory E Gonye

The present invention relates to the generation and use of a cellular array or a cellular array in combination with other genome-registered arrays (an array of arrays) for the determination of gene function and/or perturbation mode of action. Each cellular array consists of a number of microbial strains. Each strain comprises one reporter gene fusion made up of a gene or gene fragment operably linked to a reporter gene. Each gene or gene fragment has been &ldquo;registered&rdquo; or mapped to a specific location in the genome of the organism. The genome-registered collection of the invention may be used to determine alterations in gene expression under a variety of conditions. Such collections are amenable to rapid assay and may be used to confirm, correct or augment data generated from DNA micro array technology.

Cellular arrays for the identification of altered gene expression

A novel method of identifying genetic markers linked to alleles conferring yield potential of a crop species has been developed. By conducting genetic marker analysis of a set of current elite lines and the ancestral population from which they were derived by decades of plant breeding, one can determine and compare the expected and observed allele frequencies within elite populations at numerous polymorphic loci. Since the traditional plant breeding effort has consistently utilized yield as a selection criteria, deviations from expected allele frequency at certain loci have been used to identify alleles that confer yield potential. Agronomically superior progeny can, therefore, be selected utilizing genetic markers.

Method to identify genetic markers that are linked to agronomically

A novel method of identifying genetic markers linked to allels conferring yield potential of a crop species has been developed. By conducting genetic marker analysis of a set of current elite lines and the ancestral population from which they were derived by decades of plant breeding, one can determine and compare the expected and observed allele frequencies within elite populations at numerous polymorphic loci. Since the traditional plant breeding effort has consistently utilized yield as a selection criteria, deviations from expected allele frequency at certain loci have been used to identify alleles that confer yield potential. Agronomically superior progeny can, therefore, be selected utilizing genetic markers.

Growing community of inventors

Wilmington, DE, United States of America

Michael K Hanafey