Massachusetts Institute of Technology

University of Minnesota

Michael Joseph Smanski

Christopher A Voigt

Dehua Zhao

Maciej Maselko

Szu-Yi Hsu

The invention relates to methods and products for generating diverse libraries of genetic material. The products include libraries and constructed nucleic acids as well as kits and databases and systems thereof.

Directed evolution of synthetic gene cluster

This disclosure describes a programmable transcription factor system. Generally, the system includes a programmable transcription factor, a polynucleotide that includes a coding region whose expression is either required or toxic for cell viability, and a promoter operably linked to the polynucleotide and having a binding site for the programmable transcription factor. The programmable transcription factor generally includes a domain that specifically binds to a promoter and a domain that either activates transcription (if the polynucleotide encodes an essential product) or represses transcription (if the polynucleotide encodes a toxic product).

Programmable transcription factors and methods

This disclosure describes recombinant cells and methods for microbial biosynthesis of ent-atiserenoic acid. Thus, in one aspect, this disclosure describes a recombinant cell genetically modified to exhibit increased biosynthesis of ent-atiserenoic acid compared to a comparable control cell. In some cases, the recombinant cell can include a host cell modified to include at least one heterologous polynucleotide encoding at least one enzyme in a biosynthetic pathway that produces ent-atiserenoic acid. In some cases, the recombinant cell can include a host cell and at least one heterologous enzyme in a biosynthetic pathway that produces ent-atiserenoic acid.

Growing community of inventors

Falcon Heights, MN, United States of America

Michael Joseph Smanski