Ge Healthcare UK Limited

Cancer Research Technology Limited

Michael John Francis

Nicholas Thomas

Simon Laurence John Stubbs

Rahman Aziz Ismail

Simon L Stubbs

Anne Elizabeth Jones

Ian D Goodyer

Adrian Cushing

Jonathon Noe Pines

Jonathan Mark Kendall

The present invention relates to a cytochrome c-reporter fusion protein construct comprising a modified cytochrome c protein which targets the mitochondria and has a reduced ability to induce apoptosis in a living cell. The invention also relates to nucleic acid constructs encoding such protein fusions and cells stably transfected with such constructs. The stably transfected cells of the invention can be used in assays to detect apoptosis.

Cytochrome c protein and assay

The invention provides a novel, non-destructive and dynamic process for determining the cell cycle position of living cells. The invention also provides DNA constructs, and cell lines containing such constructs, that exhibit activation and deactivation of a detectable reporter molecule in a cell cycle specific manner. The invention thus allows greater precision in determining cell cycle phase status than existing techniques and further provides a method for continuous monitoring of cell cycle progression in individual cells.

Method for determining cell cycle position

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South Glamorgan, United Kingdom

Michael John Francis