Siemens Aktiengesellschaft

Earlybio Gmbh

Michael Johannes Helou

Oliver Hayden

Sandro Francesco Tedde

Lukas Richter

Mathias Reisbeck

Gerald Warnat

The invention relates to a cartridge () for a magnetic flow cytometer, mainly extending in a x-y-plane, with an inlet () for injecting a sample () into the cartridge (), a blister () for a buffer solution () with magnetic markers to mark pregiven particles (') of the sample (), an outlet, and a fluid channel (), the fluid channel () comprising a first part that connects the inlet () with the blister () and a second part that connects the first part with the outlet, wherein the second part of the fluid channel () comprises an enrichment zone () with mechanical guiding structures to focus marked particles (&#x2032;) of the sample () in a predetermined subsection of the fluid channel () and a measuring zone () between the enrichment zone () and the outlet, the measuring zone () comprising a magnetic field sensor () in the predetermined subsection of the fluid channel () in order to provide simplified and accelerated means for measuring particles, in particular concentrations of particles, of a sample.

Cartridge for a magnetic flow cytometer, a magnetic flow cytometer, and method for analysing a sample with such a cartridge

The invention relates to a device and a method for fluidic cell guidance for flow cytometry or analyte enrichment. This allows magnetically marked analytes, in particular cells (), to be dynamically enriched and individually detected in the flow from a sample, in particular magnetoresistively. For cell guidance, guiding ridges () are arranged in a flow channel (), and so, in addition to a magnetic enrichment force () and the shearing force of the flow (), a deflecting force () caused by the fluidic obstacles () also acts on the cells () to be detected.

Fluidic cell guidance for flow cytometry

The disclosure relates to flow cytometry. A method for precise individual cell detection and cell measurement of cells in the flow is disclosed. A pair of magnetoresistive components are used to produce a characteristic measuring signal profile from which the following information can be obtained: number of measurement deviations, measurement deviation distances, measurement deviation amplitudes, measurement deviation direction and measurement deviation direction sequence. The flow speed and the cell diameter can also be determined. Also, the signal noise ratio can be determined using the measurement deviation amplitude.

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Wuppertal, Germany

Michael Johannes Helou