Other

Incyte Pharmaceuticals, Inc.

Incyte Genomics, Inc.

Incyte Corporation

Michael J Rose

Karl J Guegler

Ruoying Tan

Douglas R Savor

Methods and compositions are provided for producing full-length cDNA libraries. In the subject methods, full length first strand cDNAs are isolated using a fusion protein of an eIF-4E domain and an eIF-4G domain separated by a flexible linker. Also provided is the novel fusion protein employed in the subject methods, as well as nucleic acids encoding, and host cells capable of expressing, the same. Finally, kits for use in practicing the subject methods are provided. The subject invention finds use in a variety of applications in which full-length cDNA libraries are employed.

Nucleic acid encoding a fusion protein comprising an EIF-4E domain and an EIF-4G domain joined by a linker domain

Methods and compositions for producing full length cDNA libraries

Fusion protein comprising an eIF-4E domain and an eIF-4G domain joined by a linker domain

Methods and compositions are provided for producing full-length cDNA libraries. In the subject methods, full length first strand cDNAs are isolated using a fusion protein of an eIF-4E domain and an eIF-4G domain separated by a flexible linker. Also provided is the novel fusion protein employed in the subject methods, as well as nucleic acids encoding, and host cells capable of expressing, the same. Finally, kits for use in practicing the subject methods are provided. The subject invention finds use in a variety of applications in which full-length CDNA libraries are employed.

Methods are provided for preparing a double-stranded cDNA corresponding to the 5' end of an mRNA. In the subject methods, an mRNA is first contacted with an oligo dT primer under first strand cDNA synthesis conditions. Next, the resultant hybrid is contacted with a random primer under first strand cDNA synthesis conditions, such that a cDNA complementary to the 5' end of the mRNA is produced. The resultant hybrid molecule is converted to two different double-stranded cDNAs, a first cDNA comprising the oligo dT primer and a second cDNA lacking the oligo dT primer. The two double-stranded cDNAs are then separated from each other. The subject methods find use in a variety of applications, and find particular use in the synthesis of 5' enriched cDNA libraries. Also provided are cDNA libraries produced by the subject methods, as well as kits for performing the subject methods.

Methods and compositions for producing 5' enriched cDNA libraries

A standard digital display alarm clock is provided which is driven by a 1.5 volt DC power source. A two-tone alarm device, whose sound level is adjustable from 60 dB to 100 dB, is electrically wired to the clock and sounds when the clock attains a predetermined time. The power for the alarm device is supplied by either a 9 volt DC source contained within the clock or a 12 volt DC automotive cigarette lighter. The clock is provided with an intermittent alert feature which will cause the alarm to sound every four minutes for an indefinite amount of time and requires operator input to deactivate.

Alarm and intermittent alert system for vehicle operation

Caps of the type used by baseball players but also used by other athletes and their fans have an opening at the back. A strap is attached to the headband of the cap on either side of the opening and the straps can be used to adjust the cap to fit the head size of the wearer by overlapping the ends of the straps to the necessary extent. One strap usually has spaced holes and the other spaced studs which fit into the holes. The present invention provides a panel which may carry the logo of a team, a merchandiser's advertisement or other design. The panel is secured to the overlapped straps in various ways described in detail.

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Palo Alto, CA, United States of America

Michael J Rose