Tm Technologies, Inc.

Other

The State University of New York

Luminex Molecular Diagnostics, Inc.

Molecular Staging Inc.

Michael J Lane

Brian D Faldasz

Albert S Benight

David C Ward

Patricia Bray-Ward

Gyanendra Kumar

Jerrie Gavalchin

The invention provides reagents and methods for lateral flow assays and quantitative capture or determination of components, including cells, in a sample. In one aspect, reagents and methods for diagnostic assay are provided. In one embodiment an assay for determining T cell numbers, particularly a CD2+ CD4+ T cell assay is provided. A manufacturing method for producing rapid diagnostic assays in a decentralized manner is also described. The method generates net economic advantages over conventional diagnostic manufacturing practices.

Lateral flow based methods and assays for rapid and inexpensive diagnostic tests

A concrete form with a keyway with an opening between opposing keyway lips together with a base that fits through the keyway opening and rotates without restriction in the keyway such that a portion of the base rests behind the keyway lips so when it is pulled as a clamp or tie is exercised to secure a stake to the form the base is secured tight against the form front. The concrete form further has at least one brace wall extending between intermediate the keyway back wall and the form back. Form end connectors securable in form ends are provided that join two forms aligned end to end.

Concrete form with keyway and clamp with base engaging the keyway

The invention relates to the field of molecular biology, nucleic acid chemistry and medical diagnostics. More specifically, it relates to methods and compositions for promoting the hybridization of a nucleic acid probe with a target nucleic acid sequence which is not perfectly matched to the probe.

Methods and compositions for modulating 'marginally indiscriminant' hybridizations

Disclosed is a method and compositions for the sensitive detection of the amount and location of specific nucleic acid sequences. The method makes use of a branched oligomer, referred to as a lollipop oligomer, that has a tail portion, a right arm portion, and a left arm portion. These three components are joined at a common junction making a three-tailed structure. The two arms each end with sequences complementary to adjacent sequences in a target sequence. This allows the right and left arms to be ligated together when the oligomer is hybridized to the target sequence, thus topologically linking the oligomer to the target sequence. The tail portion can then be detected at the location of the target sequence.

Signal amplification with lollipop probes

Methods for detecting the presence or absence of an analyte in a sample are disclosed. Kits for performing the analysis methods of the invention are also disclosed.

Signal amplification method

Methods, compositions, and kits for modulating the stability of at least one nucleic acid duplex, are disclosed.

Methods and compositions for modulating melting temperatures of nucleic acids

Methods for identifying a site on a nucleic acid sequence having a desired free energy variability are disclosed. The methods are useful for, e.g., selecting antisense oligonucleotides, e.g., for therapeutic use.

Process for selecting anti-sense oligonucleotides

A method of providing the sequence of a single stranded nucleic acid molecule, which, when hybridized to a complementary single stranded molecule, results in a double stranded (duplex) structure having a preselected value for a free energy parameter.

Thermodynamics, design, and use of nucleic acid sequences

Nucleic acid capture moieties, methods of using nucleic acid capture moieties, reaction mixtures including nucleic acid capture moieties, and kits including nucleic acid capture moieties are disclosed.

Nucleic acid capture moieties

A method for determining at least one DNA sequence that can be employed as a flanking sequence to a DNA binding site for a ligand, wherein the DNA binding site is located within a preselected DNA sequence, to provide a relative increase, decrease, or approximate equality in binding constant of the ligand for its DNA binding site, or relative composite reaction rate for the ligand and a DNA sequence. Also provided are applications of the method to determine DNA primers useful in conjunction with DNA amplification to detect the presence or absence of DNA sequences and to diagnose diseases related to viral DNA, such as human immunodeficiency virus.

Growing community of inventors

Baldwinsville, NY, United States of America

Michael J Lane