Agilent Technologies, Inc.

Michael J Kimzey

Francis T Haxo

Alexander Gyenes

Vaishali Sharma

Sergey Vlasenko

Fielito U Limjap

The invention relates to an activated form of procaine, and the use of the activated procaine, or salts or solvates thereof, to label amine-containing compounds, such as N-glycans, amine-containing amino acids, amine-containing peptides, amine-containing proteins, or other amine-containing compounds in a sample. Use of activated procaine as a label allows for sensitive detection of compounds labeled with it by both fluorescence and mass spectrometry.

Methods for labeling amine-containing compounds

The presence of mild electrophiles, such as aldehydes, during the denaturation of glycoproteins or glycopeptides and subsequent enzymatic deglycosylation reduces artifacts in subsequent analyses of the glycans released from the glycoproteins or glycopeptides.

Kits comprising aldehydes to reduce artifacts in analyzing glycans released from glycoproteins or glycopeptides

The present disclosure provides methods of improving the resolution of labeled glycans in capillary electrophoresis techniques using a gel as a sieving matrix, by incorporating polyamines in the gel.

Use of polyamines as resolving agents for capillary electrophoresis of labeled glycans using gels

Ammonium cation detergents comprising a quaternary or tertiary ammonium cation can be used as detergents to denature proteins and are particularly useful in denaturing glycoproteins or glycopeptides prior to enzymatic deglycosylation. Ammonium cation detergents with sulfate or sulfonate anions are particularly useful.

Solutions of quaternary and tertiary ammonium cation detergents to denature proteins, and kits for making them

The present disclosure provides methods of improving the resolution of analytes by in electrophoretic separations using a gel by incorporating into the gel an effective amount of one or more tri- or tetra-hydroxyl quaternary ammonium compounds, or a mixture of such compounds.

Use of tri- and tetra-hydroxyl quaternary ammonium compounds as resolving agents for electrophoretic separations

Use of quaternary and tertiary ammonium cations to denature proteins

Bispyridines improve the labeling of nucleophiles, including amines and thiols and are particularly useful for improving labeling with acidic and basic labels. Use of bispyridines with such labels dramatically increases labeling compared to protocols without a bispyridine. The labeled nucleophile can then be subjected to standard analytical methods.

Use of bispyridines to improve labeling of nucleophiles

Use of mild electrophiles to reduce artifacts in analyzing glycans released from glycoproteins or glycopeptides

The invention relates to an activated form of procaine, and the use of the activated procaine, or salts or solvates thereof, to label amine-containing compounds, such as N-glycans, amine-containing amino acids, amine-containing peptides, amine-containing proteins, or other amine-containing compounds in a sample. Use of activated procaine as a label allows for sensitive detection of compounds labeled with it both by fluorescence and by mass spectrometry.

Growing community of inventors

Laguna Hills, CA, United States of America

Michael J Kimzey