Biovest International, Inc.

Genmab A+s

Cellex Biosciences, Inc.

Michael J Gramer

Grant A Adams

Robert J Wojciechowski

Mark Hirschel

Darrell Paul Page

Thiem Chan Duong Wong

Martin Peder Crep

Scott Waniger

Karl Patrick Bongers

Beverly Norris

Paul Parren

Janine Schuurman

Aran Frank Labrijn

Arnout F Gerritsen

Joyce Meesters

Joost J Neijssen

Patrick Van Berkel

Ewald T J Van Den Bremer

Amitava Kundu

Muriel Van Kampen

Patrick Priem

Werner L Vos

Joyce I Meesters

Ewald TJ Van Den Bremer

The present invention relates to an in vitro method for production of heterodimeric proteins.

Production of heterodimeric proteins

A cell culture system for the production of cells and cell derived products includes a reusable instrumentation base device incorporating hardware to support cell culture growth. A disposable cultureware module including a cell growth chamber is removably attachable to the instrumentation base device. The base device includes microprocessor control and a pump for circulating cell culture medium through the cell growth chamber. The cultureware module is removably attached to the instrumentation base device. Cells are introduced into the cell growth chamber and a source of medium is fluidly attached to the cultureware module. Operating parameters are programmed into the microprocessor control. The pump is operated to circulate the medium through the cell growth chamber to grow cells or cell products therein. The grown cells or cell products are harvested from the cell growth chamber and the cultureware module is then disposed.

Method and system for the production of cells and cell products and applications thereof

A 'micro' hollow fiber bioreactor and method of use are provided for use in screening different cell lines and process conditions. The bioreactor includes the use of an oxygen permeable (e.g., silicone rubber) tube sealably containing a hollow fiber bundle, in order to create an extracapillary space to provide a medium reservoir and an intracapillary space for the growth of cells. The bioreactor avoids the need for oxygen or medium pumps or supply systems, and permits multiple cell lines, and/or multiple conditions to be evaluated simultaneously. Preferably, the tube has an oxygen permeability of between about 100.times.10.sup.-10 to about 10,000.times.10.sup.-10 (cc-mm/sec-cm.sup.2 -cm Hg), the extracapillary space provides a medium reservoir of about 1 ml to about 100 ml, the intracapillary space provides a cell culture volume of about 0.1 ml to about 1 ml, the hollow fibers have a molecular weight cut off from about 1 kD to about 1,000 kD and a pore size of from about 0.01 microns to about 5 microns, and the tube contains about 1 to about 1000 hollow fibers.

Micro hollow fiber bioreactor

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Lino Lakes, MN, United States of America

Michael J Gramer