Gen-probe, Inc.

Michael J Gilly

James M Carrick

Xianqun Wang

Sangeetha Vijaysri Nair

Susan K Yamagata

Jeffrey D Chismar

Ankur Shah

Andrew S Gilder

Method and system for quantifying target nucleic acids using real-time amplification and internal calibration adjustment. The invention employs dual reference calibration curves for approximating a complete calibration curve from only a single adjustment calibrator amplified on the instrument that is to be calibrated.

Single-point calibration of a nucleic acid analyzer

A multiplexed nucleic acid amplification and detection system useful for detecting the presence of multiple specific nucleic acid sequences or single nucleotide polymorphisms (i.e., 'SNPs') in a temperature-dependent fashion using only a single fluorescence detection channel of a nucleic acid analyzer. The technique can be carried out using standard PCR instrumentation equipped for fluorescence detection or monitoring.

Temperature-selectable fret cassette signaling

Method of calibrating a nucleic acid analyzer

Dual reference calibration method and system for quantifying polynucleotides

System for analyzing a polynucleotide-containing sample using real-time amplification and monitoring. The system includes a computer or processing device with software for performing a vector analysis of growth curves. The vector analysis advantageously simplifies polynucleotide quantitation by circumventing the need to establish thresholds used for calculating initiation of the growth phase, or to calculate derivatives.

Real-time amplification and monitoring system incorporating vector-based algorithm for analyzing polynucleotide-containing samples

Machine executable method of analyzing growth curve data to identify the transition from a baseline phase into a growth phase. Applications of the method include analysis of results from time-dependent monitoring of amplicon synthesis in a nucleic acid amplification reaction to quantify a starting amount of a nucleic acid template in a test sample. The method advantageously simplifies the quantitation by circumventing the need to establish thresholds used for calculating initiation of the growth phase, to calculate derivatives, or to perform linear regression analysis.

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San Diego, CA, United States of America

Michael J Gilly