Wyatt Technology Corporation

Laxmi Therapeutic Devices, Inc.

Michael I Larkin

Amy D Hanlon

Daniel I Some

David N Villalpando

Steven P Trainoff

Barbara R Maurer

Jeremy W Jarrett

Thomas Metzmaker

Matthew Yavorsky

Vivianna Day

Richard J Sleiman

Stephen C Minne

Shiva Ramini

Peter G Neilson

Dallin Ostler

Roshanne Malekmadani

Scott Mallett

A monitor for determining analyte concentrations in vivo, includes a housing configured to adhere to the skin of a subject, an elongate sensor body configured to extend from the housing into the skin of the subject, an analyte sensing region positioned on the sensor body such that the analyte sensing region is configured to be held in the dermis of the skin of the subject, and at least one engagement surface configured to facilitate increased anchoring of the sensor body in the skin of the subject.

Dermal sensor for determining analyte concentrations

An applicator assembly for applying at least a portion of an analyte monitor includes a frame having a longitudinal axis and an opening, a resilient member having at least a first portion fixed relative to the frame and a second portion movable axially relative to the frame, a needle, and a cap engageable with the frame to cover at least part of the opening. The cap is adjustable to increase a bias on the resilient member and to move the second portion of the resilient member away from the opening. When the cap is separated from the frame, the second portion of the resilient member is configured to be held relative to the frame. The hold on the second portion of the resilient member is releasable, such that the bias on the resilient member advances the second portion of the resilient member and the needle towards the opening.

Applicators for glucose monitors, methods for applying glucose monitors, and glucose monitors for use with such applicators

The present disclosure describes a computer implemented method, a system, and a computer program product of purifying a sample solution via real-time multi-angle light scattering. In an embodiment, the method, system, and computer program product include receiving from a MALS instrument baseline scattering intensity values of a pure buffer, receiving from the MALS instrument scattering intensity values of a sample solution, and characterizing at least one component of the sample solution, resulting in a time series of values of a dimension, D, of the at least one component and a time series of values of excess Rayleigh ratio, R0, of the at least one component, and determining that the values of the dimension, D, fall within a dimension, D, value range and that the values of excess Rayleigh ratio, R0, fall within an excess Rayleigh ratio value range, and transmitting a collect sample solution command to collect the sample solution.

Purifying a sample solution via real-time multi-angle light scattering

The present disclosure describes a method, a system, and a computer program product of indicating a status of an analytical instrument on a screen of the analytical instrument. In an embodiment, the method, the system, and the computer program product include receiving data from an analytical instrument monitoring a liquid sample, segmenting the received data into data segments for at least two characteristics of at least one of the instrument, the sample, and an operating environment of the instrument, analyzing each of the data segments for the at least two characteristics, retrieving threshold values for the at least two characteristics from a computer data source, calculating at least one status of at least one of the instrument, the sample, and the operating environment, with respect to the analyzed data segments and the threshold values, and displaying the at least one status on a display of the instrument.

Indicating a status of an analytical instrument on a screen of the analytical instrument

The present disclosure describes a method, a system, and a computer program product of analyzing data collected by analytical instruments. In an embodiment, the method, the system, and the computer program product include receiving set-up information, running at least one incomplete analytical method on at least one known sample on at least one analytical instrument with respect to the set-up information, resulting in known sample data, processing the at least one incomplete analytical method with respect to the known sample data, resulting in at least one validated analytical method, and running the at least one validated analytical method on at least one unknown sample on the at least one analytical instrument with respect to the set-up information, resulting in analyzed sample data.

Analyzing data collected by analytical instruments

This invention enables high throughput detection of small molecule effectors of particle association, as well as quantification of association constants, stoichiometry, and conformation. Given a set of particle solutions having different concentrations, dynamic light scattering measurements are used to determine the average hydrodynamic radius, as a function of concentration. The series of average hydrodynamic radii as a function of concentration are fitted with stoichiometric association models containing the parameters of molar mass, modeled concentrations, and modeled hydrodynamic radii of the associated complexes. In addition to the average hydrodynamic radii value analysis, the experimental data may be fit/analyzed in alternate ways. This method may be applied to a single species that is self-associating or to multiple species that are hetero-associating. This method may also be used to characterize and quantify the association between a modulator and the associating species.

Method of characterizing interactions and screening for effectors

This invention enables high throughput detection of small molecule effectors of particle association, as well as quantification of association constants, stoichiometry, and conformation. 'Particle' refers to any discrete particle, such as a protein, nucleic acid, carbohydrate, liposome, virus, synthesized polymer, nanoparticle, colloid, latex sphere, etc. Given a set of particle solutions having different concentrations, dynamic light scattering measurements are used to determine the average hydrodynamic radius, r, as a function of concentration. The series of ras a function of concentration are fitted with stoichiometric association models containing the parameters of molar mass, modeled concentrations, and modeled hydrodynamic radii of the associated complexes. In addition to the rvalue analysis, the experimental data may be fit/analyzed in alternate ways. This method may be applied to a single species that is self-associating or to multiple species that are hetero-associating. This method may also be used to characterize and quantify the association between a modulator and the associating species.

A lid for a multiwell plates which allows improved light scattering measurement of liquid samples within the wells of a multiwell plate, and which at the same time mitigates evaporation from said samples is disclosed. A surface element protrudes from the bottom of the lid into the fluid in a well. The protruding element may be hollow or solid, and the beam of light, directed into the element may act to capture or direct the beam while preventing backscatter from reaching the light scattering detector or detectors. The protruding element may thus direct the beam from the well without the beam having to pass through a fluid/air interface. The angle and shape of the lid surfaces may be optimized to minimize or eliminate back-reflection. Light absorbing and/or light blocking colorization may also be employed to minimize or eliminate back reflection. Evaporation is controlled by physically capping the well with the lid, either sealing against the face at the top of the well or the inside surface of the well.

Multiwell plate lid for improved optical measurements

A lid for multiwell plates, allowing improved optical measurement of liquid samples within its wells, while mitigating evaporation from said samples, is disclosed. A surface element protrudes from the bottom of the lid into the fluid within a well. The protruding element may be hollow or solid such that light directed into the element may act to capture or direct the beam while preventing backscatter from reaching one or more detectors. The protruding element may direct the beam from the well without requiring the beam to pass through a fluid/air interface. The angle and shape of the lid surfaces and/or light absorbing/blocking colorization may be employed to minimize or eliminate back reflection. Evaporation is controlled by physically capping the well with the lid, either sealing against the face at the top of the well or the inside surface of the well.

A method and apparatus for the illumination of a sample are disclosed. An imaging illumination light source is directed to pass through an absorbing/transmitting structure in order to illuminate the sample and any containing vessel. A diffuser may aid in properly dispersing the light from the imaging illumination source. A light sensitive detector such as a camera records an image therefrom. The beam from a light scattering source is directed through the sample and any containing vessel, and upon exiting the sample/vessel, impinges upon the absorbing/transmitting structure selected to absorb at the wavelength of the light scattering source. Scattered light from the sample is collected by a photo detector. Methods of use for the novel lighting system are also disclosed.

Method and apparatus to illuminate sample and containing vessel in a light scattering detector

An improved cell for a walk-off refractometer is disclosed that permits measurement of the differential refractive index, DRI, between a sample fluid and a reference fluid. In addition, the new cell design permits the measurement of the refractive index, RI, of a fluid relative to the refractive index of the material comprising or surrounding the flow cell. Thus a single instrument may be used to measure separately the RI of a sample fluid and the DRI between a sample fluid and a reference fluid. The new flow cell contains two chambers, typical of a DRI instrument, but an asymmetric internal angle in either the sample or the reference chamber. By the provision of this unique structure, it is an objective of this invention to be able to measure the refractive index of a fluid relative to the refractive index of the material comprising the flow cell or relative to the medium surrounding the flow cell, either of which may be considered a measurement of the RI of the fluid. With the addition of mirror means, it is the further objective of this invention to improve its sensitivity. A further objective of the invention is to measure the asymmetric internal angle of the flow cell using well-characterized reference fluids.

Refractometer cell for both absolute and differential refractive index measurement of fluids

An improved differential refractometer incorporating a photodetector array is disclosed. Using a multi-element photo array provides the basis for measurement of differential refractive index values with a heretofore unattainable combination of sensitivity of measurement and concurrent range of measurement. Within the large dynamic range attainable, the detector structure provides equal sensitivity irrespective of deflection within the range. The transmitted light beam is tailored to provide a spatial variation of the light intensity at the array improving thereby the precision of measurement of its displacement. This in turn results in improved precision in the reported differential refractive index and in the calculation of the differential refractive index increment dn/dc. Integrating the detector array into the flow cell structure of the parent case results in a detector of exceptional sensitivity and range for sample quantities far smaller than required by conventional differential refractometers.

Enhanced sensitivity differential refractometer incorporating a photodetector array

A sensitivity-enhanced flow cell to be used in the determination of the differential refractive index increment of a sample fluid relative to a reference fluid is disclosed. The invention permits the use of smaller sample amounts without sacrificing overall sensitivity. Equally important, said improved flow cell produces measurements of increased precision without requirement for increased sample amount. This is achieved by means of two chambers within said cell whose volumes are different. The sample fluid chamber is the smaller of the two with the reference fluid chamber constructed so that the incident illumination beam, upon passage through said sample chamber and displacement by the partition element located therebetween said sample and reference chambers, passes through said reference chamber without grazing any of the confining walls or striking corners of said sensitivity-enhanced flow cell. As the amount of deflection of said transmitted beam depends upon the refractive indices of the fluids relative to the transparent matter of which the containing cell is comprised, as well as the RI difference between said fluids themselves, the reference chamber is designed to transmit said incident light beam without grazing degradation thereof for all practical ranges of expected fluid and cell refractive indices.

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Santa Barbara, CA, United States of America

Michael I Larkin