Lonza Cologne Ag

Lonza Cologne Gmbh

Michael Habig

Herbert Müller-Hartmann

Herbert Mueller-Hart-Mann

Herbert Mueller-Hartmann

Gregor Siebenkotten

Peter Hoffmann

The invention concerns a containerwith chamberswhich each comprise at least one pair of electrodes including a firstand a second electrodefor the application of electric voltage for generating an electric field within one chamber. At least two first electrodesof different chambersare conductively coupled and at least one second electrodeof said chamberis separately conductively connectable. The invention further concerns a method for manufacturing said containeras well as a device for electrically contacting at least one of said containers

Container and device for generating electric fields in different chambers

Disclosed is a method for treating biological material via electrical current, in which the biological material is added to a small volume of a buffer solution having relative high ionic strength. A strong electrical field is generated in the buffer solution by a high voltage pulse having a preset duration. The biological material is added to at most 50 &#x3bc;l of a buffer solution with an ionic strength of at least 100 mmol/l. By at least one voltage pulse having a preset duration of at least 10 &#x3bc;s, an electrical field with a field strength of at least 1 kV/cm is generated in the buffer solution. The voltage pulse is hereby interrupted at least once for a duration of at least 100 &#x3bc;s and is then again continued.

Method for treating small volumes with electrical current

The invention relates to a containerfor receiving an aqueous solution, which is formed at least partially by an outer limitforming an inner chamberfor receiving the solution, and which comprises at least one area which acts as an electrodewhen an electric voltage is applied and a subsequent discharge occurs, wherein at least one electrodeis made of a conductive synthetic material at least based on a plastic material which is doped with at least one conductive substance. A containerof the above-mentioned kind is created this way, which is simple and economical to produce and also, for example, enables an efficient transfection of living cells by means of electroporation or an effective electrofusion.

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Basel, Switzerland

Michael Habig