USA As Represented by Secretary of the Navy

Michael H Stewart

Kimihiro Susumu

Igor L Medintz

Eunkeu Oh

Jeffrey R Deschamps

James B Delehanty

Philip E Dawson

Alan L Huston

Hedi M Mattoussi

Alexander L Efros

Darryl A Boyd

Okhil Nag

Lauren D Field

James P Wissman

W Russ Algar

Rhonda Michele Stroud

Steven C Erwin

Juan Bautista Blanco-Canosa

Kelly Boeneman

Glyn Dawson

Joseph G Tischler

Joyce A Breger

Paul D Cunningham

Scott Walper

Dorothy Farrell

Sarah F Brittman

Parag Chitnis

Janice E Boercker

Patrick Y Yee

John R Cressman

Ryan Walters

Bing C Mei

Nashaat Rasheed

Chase T Ellis

This disclosure concerns a method of making a ligand for Quantum Dot functionalization, a method of making a functionalized Quantum Dot (QD) with a ligand, and a method of making a transparent luminescent quantum dot thiol-yne nanocomposite with tailorable optical, thermal, and mechanical properties. The prepolymer solution and functionalized Quantum Dot can be used in additive manufacturing.

Fabrication of luminescent quantum dot thiol-yne nanocomposites with tailorable optical, thermal and mechanical properties

A nanoplatelet serves as a substrate for immobilizing enzymes involved in consecutive reactions as a cascade. This results in a significant increase in the rate of catalysis as well as final product yield compared to non-immobilized enzymes or enzymes immobilized to quantum dots.

Self-assembled nanoplatelet-enzyme bioconjugates providing for increased biocatalytic efficiency

A process for synthesizing CuS/PbS core/shell nanocrystals. Pb-oleate is mixed with 1-octadecene and heated to 60&#xb0; C. CuS core solution and bis(trimethylsilyl)sulfide stock solution are added and the mixture is stirred at 60&#xb0; C. for 6 minutes to form the PbS shell around the CuS nanocrystal cores. The flask is cooled and acetonitrile and toluene is added and the mixture is centrifuged to precipitate and remove the CuS/PbS core/shell nanocrystals from the reaction mixture. The reaction also produces homogeneously nucleated PbS nanocrystals, which are removed from the CuS/PbS core/shell reaction mixture via size-selective precipitation. By tailoring the amounts of Pb-oleate and bis(trimethylsilyl)sulfide stock solution in the reaction vessel, while maintaining their molar ratio of 1.5:1 and the number of CuS cores in the reaction, CuS/PbS core/shell nanocrystals having a predetermined shell thickness of PbS, and thus a predetermined level of chemical stability, can be obtained.

Core/shell nanocrystals with copper sulfide cores and lead sulfide shells

A construct for detecting cellular membrane potential includes a nanoparticle operable as an electron donor; a modular peptide attached to the nanoparticle, the peptide comprising a nanoparticle association domain, a motif configured to mediate peptide insertion into the plasma membrane, and at least one attachment point for an electron acceptor positioned at a controlled distance from the nanoparticle; and an electron acceptor. The nanoparticle can be a quantum dot and the electron acceptor can be Cfullerene. Emission correlates with cellular membrane potential.

Modular, multifunctional nanoparticle-based bioconjugate for realtime visualization of cellular membrane potential

A construct for detecting cellular membrane potential includes a nanoparticle operable as an electron donor; a modular peptide attached to the nanoparticle, the peptide comprising a nanoparticle association domain, a motif configured to mediate peptide insertion into the plasma membrane, and at least one attachment point for an electron acceptor positioned at a controlled distance from the nanoparticle; and an electron acceptor. The nanoparticle can be a quantum dot and the electron acceptor can be Cfullerene. Photoacoustic emission from the construct correlates with cellular membrane potential.

Multifunctional nanoparticle bioconjugates for photoacoustic-based recording of cellular membrane potential

A ligand design allows compact nanoparticle materials, such as quantum dots (QDs), with excellent colloidal stability over a wide range of pH and under high salt concentrations. Self-assembled biomolecular conjugates with QDs can be obtained which are stable in biological environments. Energy transfer with these ligands is maximized by minimizing distances between QDs/nanoparticles and donors/acceptors directly attached to the ligands or assembled on their surfaces.

Compact multifunctional ligand to enhance colloidal stability of nanoparticles

Quantum dots are modified with varying amounts of (a) a redox-active moiety effective to perform charge transfer quenching, and (b) a fluorescent dye effective to perform fluorescence resonance energy transfer (FRET), so that the modified quantum dots have a plurality of photophysical properties. The FRET and charge transfer pathways operate independently, providing for two channels of control for varying luminescence of quantum dots having the same innate properties.

Simultaneous modulation of quantum dot photoluminescence using orthogonal fluorescence resonance energy transfer (FRET) and charge transfer quenching (CTQ)

A peptide attached to a nanoparticles (such as quantum dots) selectively directs the nanoparticles to neurons in a tissue or organism.

Delivery of nanoparticles to neurons

Disclosed herein are the compounds shown below and methods of their synthesis. The value m is a positive integer. R comprises an alkyl chain or an alkoxy chain. Each X comprises a metal binding group. Each E is a methoxy group or comprises a biomolecule reactive group or a residue thereof. E optionally comprises a protecting group. The value n is a positive integer. The value p is zero or one. Y is OCH, OH, NH, or COOH. 

Multifunctional metal-chelating ligands

Compounds having the formulas below. The values n and x are independently selected positive integers. 

Metal oxide-chelating ligands

Growing community of inventors

Springfield, VA, United States of America

Michael H Stewart