E.i. Dupont De Nemours and Company

Bio-technical Resources, Inc.

Michael H Reimer

James R Millis

Alan D Grund

Phillip Terry Olson

Ronald J Huss

A process for the recovery of high purity natamycin from fermentaion broth by extraction with methanol is disclosed. In one embodiment the process comprises the steps:

Natamycin recovery

A process for dehydrating compounds of the formula ##STR1## where --R is --CH.dbd.CH.sub.2, --C.tbd.CH --C.tbd.N or ##STR2## in an aqueous solution thereof is disclosed. In the case where R is --CH.dbd.CH.sub.2 or --C.dbd.CH, the temperature is preferably at least 50.degree. C. and preferably 75.degree. to 100.degree. C. The base is at least 0.1N and preferably 1.0 to 8N. Preferred bases are NaOH, KOH and C.sub.5 OH. In the case where --R is C.tbd.N, the temperature preferably is 20.degree. to 50.degree. C.

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Sheboygan, WI, United States of America

Michael H Reimer