Monsanto Technology LLC

Dekalb Genetics Corporation

University of Missouri

Michael H Luethy

Thomas M Malvar

Shihshieh Huang

Dale A Voyles

Alan L Kriz

Rebecca A Kelly

Mark A Dizigan

Kathleen P Malloy

Douglas D Randall

Jan A Miernyk

Mark L Johnston

Brian P Mooney

Larry A Gilbertson

James Kevin Roberts

Edwards M Allen

David K Kovalic

Yuanji Zhang

Linda L Lutfiyya

Liang Guo

Sara Elizabeth Heisel

Philip W Miller

Nancy M Houmard

Alessandra Frizzi

Anti-sense-oriented RNA gene suppression agents in the form of a loop of anti-sense-oriented RNA is produced in cells of transgenic organisms, e.g. plants, by transcription from a recombinant DNA construct that comprises in 5' to 3&#x2032; order a promoter element operably linked to more than one anti-sense-oriented DNA element and one or more complementary DNA elements.

Enhanced zein reduction in transgenic corn seed

Anti-sense-oriented RNA gene suppression agents in the form of a loop of anti-sense-oriented RNA is produced in cells of transgenic organisms, e.g. plants, by transcription from a recombinant DNA construct which comprises in 5' to 3&#x2032; order a promoter element operably linked to an anti-sense-oriented DNA element and a complementary DNA element.

Recombinant DNA for gene suppression

Disclosed herein are assays for detecting the presence of a lysine-increasing transgenic event based on the DNA sequence of the exogenous DNA construct inserted into the maize genome and of genomic sequences flanking the insertion site. Also provided are transgenic plants having a novel exogenous DNA construct that expresses a dihydrodipicolinic acid synthase, the activity of which results in increased lysine in a plant or plant product.

High lysine maize compositions and methods for detection thereof

Methods and compositions for the expression of transgenes in monocot plants including maize are disclosed. In the invention, gene silencing is avoided by use of monocot-homeologous sequences from plants of the genusfor transformation. Included in these transgene sequences arepromoters, enhancers, coding sequences and terminators. Suitable alternatives to maize-derived transgenes are desirable for expression in maize in that homology-based gene silencing can limit or effectively eliminate transgene expression.

Methods and compositions for expression of transgenes in plants

The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.

Engineered miRNA precursors for suppressing target gene expression in plants

The present invention provides a transgenic maize plant having in its genome transgenic DNA including sequence for zein reduction and sequence for lysine biosynthesis, whereby expression of the transgenic DNA results in a synergistically increased lysine content of seed of the transgenic maize plant. The invention further provides a method for providing maize seed with synergistically increased lysine content.

Maize seed with synergistically enhanced lysine content

The invention provides isolated gamma coixin promoters useful for the expression of heterologous coding sequences in transgenic plants. Further provided by the invention are transgenic plants comprising such promoters, expression constructs for the introduction of heterologous coding sequences into plants, and methods of using the plants and nucleic acids provided by the invention.

Disclosed herein are novel DNA constructs encoding dihydrodipicolinic acid synthase that are useful for generating transgenic events. Also provided are transgenic plants harboring such DNA constructs, the expression of which results in increased lysine in the plant or plant product. Also provided are maize plants having LY038 Event and progeny thereof having this event. Additionally disclosed are assays for detecting the presence of a lysine-increasing transgenic event based on the DNA sequence of the exogenous DNA construct inserted into the maize genome and of genomic sequences flanking the insertion site.

High lysine maize compositions and event LY038 maize plants

Growing community of inventors

Mystic, CT, United States of America

Michael H Luethy