Gen-probe, Inc.

Michael Garth Riggs

Mathoor Sivaram

Starla Dianne Tudor

Daniel L Kacian

James Garfield Putnam

Matthew Sorensen

A recombinant plasmid for expression of Moloney Murine Leukemia Virus (MMLV)-derived reverse transcriptase in cells deficient in the expression of RNAse activity, a method for purification of the recombinant enzyme, and a purified recombinant reverse transcriptase for suitable use in cDNA and nucleic acid amplification procedures are disclosed.

Recombinant DNA encoding a reverse transcriptase derived from moloney murine leukemia virus

Composition and methods for the expression of recombinant DNA polymerase enzymes derived from Bacillus stearothermophilus. The present invention also concerns methods for purifying recombinant Bst DNA polymerase enzymes compositions containing the purified enzymes in a form suitable for conducting biochemical reactions, and methods for using the purified enzymes.

Purified DNA polymerase from bacillus stearothermophilus ATCC 12980

Composition and methods for the expression of recombinant DNA polymerase enzymes derived from Bacillus stearothermophilus. The present invention also concerns methods for purifying recombinant Bst DNA polymerase enzymes, compositions containing the purified enzymes in a form suitable for conducting biochemical reactions, and methods for using the purified enzymes.

Purified DNA polymerase from Bacillus stearothermophilus

A plasmid for expression of Moloney Murine Leukemia Virus-derived reverse transcriptase in E. coli cells deficient in the expression of indiginous RNAse activity, a method for purification of the recombinant enzyme, and a composition comprising a cloned and purified reverse transcriptase opimized for use in cDNA and nucleic acid amplification procedures.

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San Diego, CA, United States of America

Michael Garth Riggs