Wisconsin Alumni Research Foundation

Michael G Thomas

Jo E Handelsman

Michelle Renee Rondon

Angela M Podevels

Andrew David Berti

Yolanda A Chan

Yolande Alia Chan

Elizabeth Anne Felnagle

Sarah G Ozanick

Novel extender units for Type I polyketide synthases are provided. Also provided are genes, compounds, and methods for generating these units, and for incorporation of the novel extender units into polyketides for the purpose of generating new structural derivatives of polyketide-containing products.

Type I polyketide synthase extender units

Provided are nucleic acid molecules comprising at least a functional fragment of the capreomycin biosynthetic gene cluster, polypeptides encoded by the cluster and recombinant host cells transformed with any of the nucleic acid molecules disclosed herein. Various methods using any of the vectors or expression cassettes that encode one or more of the gene products of the cluster are provided for heterologous production of capreomycin and capreomycin derivatives.

Heterologous production of capreomycin and generation of new capreomycin derivatives through metabolic engineering

The invention provides a nucleic acid molecule comprising at least a functional fragment of the viomycin biosynthetic gene cluster, functional proteins encoded by the cluster, expression cassettes and recombinant host cells comprising a functional fragment of the viomycin cluster, and methods for generating biologically active agents using the nucleic acid molecules of the present invention.

Metabolic engineering of viomycin biosynthesis

Growing community of inventors

Madison, WI, United States of America

Michael G Thomas