University of Pennsylvania

New York University

Heritage Power LLC

Mauk Et Al.

Michael G Mauk

Haim H Bau

Changchun Liu

William R Abrams

Xianbo Qiu

Daniel Malamud

Eran Geva

Do Young Byun

Robert Hart

Zongyuan Chen

Jinzhao Song

Dharma Teja Varapula

Sirisha Phanithra Gutta

Sudhir Ramprasad

Dafeng Chen

Jason Kwa

Daneil Malamud

Xianbo Ceiu

Amanda W Dyson

Disclosed herein are methods for multiple stage isothermal amplification of nucleic acid comprising a first substantially isothermal amplification reaction on the nucleic acid to generate a first amplification product and at least one substantially isothermal amplification reaction on the first amplification product to generate at least one second amplification product in an amount sufficient for recovery, testing, or characterization.

Multiple stage isothermal enzymatic amplification

The present disclosure provides devices and methods using separating a fluid&#x2014;e.g., plasma or serum&#x2014;from whole blood. In some embodiments, the devices and methods use hydrophobic or superhydrophobic surfaces to encourage whole blood to contact a selective membrane that extracts the desired fluid component from the blood.

Fluid separator for point of care molecular diagnostics

A testing device that integrates nucleic acid isolation, isothermal amplification and detection wherein a sample and reagents are transported through a capture/binding membrane by capillary forces and the positioning of a slidebar that supports said binding membrane. Flow control and fluid actuation are effected by the position of said slidebar which makes a fluidic connection through the capture membrane situated between a reservoir of liquid on the device and an absorbing pad, and where a sequence of operations for sample loading, washing, optionally amplification, and optical detection of analytes can be done conveniently and rapidly at the point of care. With minimal operator interaction, this device can lyse, isolate, amplify, and detect nucleic acids from clinical specimens and environmental samples.

Capillary-action microfluidic device for point-of-care diagnostics

A plasma separator includes a vertical chamber with a membrane or membranes partially or fully covering the internal chamber walls and forming an inner cavity to accommodate whole blood. The plasma separator further includes a plasma collection space separated from the inner cavity with one or more membranes and a plasma removal port.

Plasma separator apparatus and associated methods

Provided are devices adapted to isolate, amplify, and detect nucleic acids that may be present in a biological sample. The devices can, in some embodiments, isolate, amplify, and detect nucleic acid in a single chamber. In other embodiments, the devices are adapted to isolate and amplify nucleic acids in a reaction chamber, after which the nucleic acids may be communicated to a pervious medium&#x2014;such as a lateral flow strip&#x2014;where the user may label and detect the nucleic acids.

Isothermal nucleic acid amplification reactor with integrated solid state membrane

Disclosed are integrated devices capable of performing a polymerase chain reaction within a single vessel. Also disclosed are related methods of sample analysis.

Integrated PCR reactor for cell lysis, nucleic acid isolation and purification, and nucleic acid amplication related applications

Provided are disposable, moisture-activated, self-heating cartridges useful for, e.g., isothermal nucleic acid amplification, incubation, and thermal actuation, and visual fluorescent detection of the amplification products. These devices may be self-contained and do not require any special instruments to operate.

Moisture-activated self-heating analysis device

Disclosed are preloaded analysis modules comprising reagents disposed within a barrier material capable of liberating the reagents, at a time advantageous to a reaction scheme, when exposed to certain activation conditions or reagents. Also disclosed are related methods for analyzing samples with such modules.

Reaction chamber having pre-stored reagents

Disclosed are mechanically-actuated devices for transporting fluids within a microfluidic circuit and performing diagnostic operations on a sample. Also disclosed are related methods for performing sample analysis effected by the motion of an actuator proximate to a microfluidic system.

Mechanically actuated diagnostic device

A structure including at least one layer of germanium formed on a surface of a ceramic substrate is provided. The layer of germanium has a thickness of not larger than 10 microns and includes grains having grain size of at least 0.05 mm. A structure including at least one layer of germanium formed on a surface of a ceramic substrate and having at least one capping layer formed on a surface of the layer of germanium is also provided. In addition, a method of forming a thin film germanium structure is provided including forming at least one layer of germanium on a surface of a ceramic substrate, then forming at least one capping layer on a surface of the layer of germanium, followed by heating and then cooling the layer of germanium.

Growing community of inventors

Greenville, DE, United States of America

Michael G Mauk