E.i. Dupont De Nemours and Company

Butamax Advanced Biofuels LLC

Butamax (tm) Advanced Biofuels LLC

Michael G Bramucci

Vasantha Nagarajan

John Edward Gavagan

Robert Donald Fallon

Mario W Chen

Manjari Singh

Stuart M Thomas

Natalia Sedkova

Kristy N Kostichka

Helene Marie Albertson-Kane

Robert DiCosimo

Mark Scott Payne

Qiong Cheng

Lori Ann Maggio-Hall

Lech Wilczek

Andrew C Eliot

Frank Edward Herkes

Charles Edwin Nakamura

Robert Dicosimo

Sarita Chauhan

Robert A Larossa

Pierre E Rouviere

Dennis Flint

Mark J Nelson

Jean-Francois Tomb

Tina K Van Dyk

Der-Ing Liao

Edward S Miller, Jr

Patricia C Brzostowicz

Carol M McCutchen

Dana R Smulski

Luan Tao

Helene M A Kane

Edward S Miller Jr

From a bacterial strain isolated from an environmental sample, after enrichment in medium containing 1-butanol as the carbon source, a new enzyme with butanol dehydrogenase activity was identified. The enzyme can convert butyraldehyde to 1-butanol, isobutyraldehyde to isobutanol, as well as 2-butanone to 2-butanol and thus is useful for biosynthesis of butanol in recombinant microbial hosts producing these substrates. The encoding gene, named sadB, was isolated from the strain identified as an isolate of

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Methods for the fermentative production of isobutanol is provided by the fermentative growth of a recombinant microorganism expressing a highly active ketol-acid reductoisomerase enzyme in addition to other enzymes required for conversion of glucose to isobutanol.

Fermentive production of isobutanol using highly active ketol-acid reductoisomerase enzymes

Provided herein are recombinant yeast host cells and methods for their use for production of fermentation products from a pyruvate utilizing pathway. Yeast host cells provided herein comprise reduced pyruvate decarboxylase activity and modified adenylate cyclase activity. In embodiments, yeast host cells provided herein comprise resistance to butanol and increased biomass production.

Butanol tolerance in microorganisms

Increasing tolerance to butanol in yeast has been accomplished by increasing activity of the cell wall integrity pathway. Yeast with increased expression of SLT2p, a mitogen activated protein kinase of the MAPK module of the cell wall integrity pathway had increased tolerance to isobutanol. These yeast may be used for improved butanol production.

Yeast with increased butanol tolerance involving cell wall integrity pathway

Increasing tolerance to butanol in yeast has been accomplished by increasing activity of the filamentous growth response. Yeast with increased expression of MSS11p, a transcriptional activator of the filamentous growth response pathway had increased tolerance to isobutanol. These yeast may be used for improved butanol production.

Yeast with increased butanol tolerance involving filamentous growth response

Increasing tolerance to butanol in yeast has been accomplished by increasing activity of the high osmolarity/glycerol response pathway. Yeast with increased expression of PBS2p, a mitogen activated protein kinase kinase of the MAPK module of the high osmolarity/glycerol response pathway may be used for improved butanol production.

Yeast with increased butanol tolerance involving high osmolarity/glycerol response pathway

A method for the production of 1-butanol by fermentation using a microbial production host is disclosed. The method employs a reduction in temperature during the fermentation process that results in a more robust tolerance of the production host to the butanol product.

Method for the production of 1-butanol

A method for the production of 2-butanol by fermentation using a microbial production host is disclosed. The method employs a reduction in temperature during the fermentation process that results in a more robust tolerance of the production host to the butanol product.

Method for the production of 2-butanol

bacteria having enhanced tolerance to butanols have been isolated. The bacteria are useful for the fermentative production of butanol.

Solvent tolerant microorganisms

bacteria having enhanced tolerance to butanols have been isolated. The bacteria are useful for the fermentive production of butanol. New methods for the isolation of butanol tolerantare also provided.

Solvent tolerant microorganisms and methods of isolation

A microbial consortium for the biodegradation of sulfonated aliphatic-aromatic co-polyesters having greater than 60 mol percent aromatic acid content based on the total acid content of the co-polyester, created by applying selective pressure. Methods to biodegrade sulfonated aliphatic-aromatic co-polyesters using microbial consortium SPDC-1 are provided.

Method to enhance biodegradation of sulfonated aliphatic-aromatic co-polyesters by addition of a microbial consortium

Growing community of inventors

Boothwyn, PA, United States of America

Michael G Bramucci