Novartis Ag

Seqirus UK Limited

Glaxosmithkline Biologicals Sa

Michael Franti

Peter W Mason

Philip R Dormitzer

Juerg Hunziker

Pirada Suphaphiphat

David Morrissey

Heidi Trusheim

Anders Lilja

Bjoern Keiner

Björn Keiner

Melissa Sackal

Rebecca Loomis

Mikhail Matrosovich

François Jean-Charles Natt

Stefania Crotta

Francois Natt

Jennifer Uhlendorff

The invention relates to platforms for delivery of herpes virus proteins to cells, particularly proteins that form complexes in vivo. In some embodiments these proteins and the complexes they form elicit potent neutralizing antibodies. Thus, presentation of herpes virus proteins using such platforms permits the generation of broad and potent immune responses useful for vaccine development.

Antigen delivery platforms

This disclosure provides platforms for delivery of herpes virus proteins to cells, particularly proteins that form complexes in vivo. In some embodiments these proteins and the complexes they form elicit potent neutralizing antibodies. Thus, presentation of herpes virus proteins using the disclosed platforms permits the generation of broad and potent immune responses useful for vaccine development.

The invention provides various reverse genetics systems for producing segmented RNA viruses, wherein the systems do not require bacteria for propagation of all of their expression constructs.

Reverse genetics systems

Methods for producing reassortant viruses are provided wherein the transcription and/or translation of the hemagglutinin and/or neuraminidase genes are suppressed.

Influenza virus reassortment method

Expression of a transgene is driven in a host cell using a pol I promoter which is not endogenous to an organism from the same taxonomic order from which the host cell is derived.

Reverse genetics using non-endogenous pol I promoters

Influenza virus reassortment

A method for rescuing a virus by reverse genetics is provided in which cells are added after transfection.

Reverse genetics methods for virus rescue

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Blainville, Canada

Michael Franti