Bioscale, Inc.

Axsun Technologies, Inc.

Proterixbio, Inc.

Michael Francis Miller

Brett P Masters

Mark Lundstrom

Dale C Flanders

Peter S Whitney

Minh Van Le

Wayne U Wang

Alok Srivastava

Alexis Sauer-Budge

Martin Arnold Schmidt

Stanley R Shanfield

Christopher C Cook

Shivalik Bakshi

Peter Wight Falb

William Matthew Dickerson

Jeffrey A Korn

David Brancazio

Lara Madison

Jaime E Arenas

Steven F Nagle

Eric A France

Eric E Fitch

David B West

Edward Marshall Alderman

Hyun-Goo Choi

Sarah Beth Hembree

Joshua R Ormsby

Vishal K Gulati

Ed Crawley

Raymond V Pourmand

Walker Sloan

In one non-limiting aspect, the invention provides a method for detecting the quality of a biological molecule comprising forming a first mixture of ingredients comprising: (i) a first binding agent that specifically binds to a tag, wherein the first binding agent is attached to a solid support; (ii) a decoy comprising a first portion comprising the tag attached to a second portion comprising an anchor; (iii) a sensor attached to a second binding agent that specifically binds to the anchor; and (iv) a sample suspected of containing a high quality biological molecule comprising a tag, wherein the tag of the high quality biological molecule is accessible; allowing interaction of the ingredients such that the sensor provides an output signal.

Methods and systems for the rapid characterization of functional biological molecules

In some aspects, an electrically responsive device can include a composite structure having spatially modulated structural properties that includes a substrate material having a surface and defining a plane; an electrically responsive material layer formed over at least a portion of the surface of the substrate material; an electrode material over portions of the electrically responsive material; and a stiffening material disposed along the electrode material, where the stiffening material has a thickness that varies and has regions of increased thickness that correspond with the regions of the composite structure along which the electrode material is disposed. The spatially modulated structural properties can include the regions of the composite structure along which the electrode material is disposed having the increased material stiffness, exclusive of the electrode material, relative to regions of the composite structure that do not include the electrode material.

Electrically responsive device

A system for processing analytes in samples includes an instrument and a cartridge. The cartridge includes fluid inputs, input and output valve assemblies, processing devices, fluid reservoirs, and channels for carrying samples from the fluid inputs to the fluid reservoirs. The valve assemblies include valves adapted to form a sealed fluid chamber in response to force applied by a movable head assembly of the instrument. Each fluid reservoir is adapted to mate and align with an air displacement pump interface member. A valve assembly includes a recess wall surrounding a recess and a valve assembly wall surrounding both the recess and the recess wall. The recess wall and the valve assembly walls are adapted to mate with and seal against a flexible sheet covering the recess, the recess wall, and the valve assembly wall. The cartridge and instrument include complementary features for finely and coarsely aligning instrument assemblies with portions of the cartridge.

Method and apparatus for analyte processing

Electrically responsive devices and methods for fabricating electrically responsive devices involves applying an electrically responsive material (e.g., an electroactive material) over at least a portion of a surface of a substrate material and applying an electrode material over at least a portion of a surface of the electrically responsive material. At least one region of the electrode material is selectively removed exposing the electrically responsive material. At least some of the electrically responsive material is selectively removed in a region corresponding to the at least one region of the electrode material.

Methods and apparatuses for determining whether a fluid has been introduced into an assay measurement apparatus involving delivering a fluid to a surface of a resonant device. The methods also involve monitoring an electrical signal output by the resonant device, wherein properties of the electrical signal vary based on physical properties of the fluid in contact with the surface of the resonant device and determining if the electrical signal output by the resonant device satisfies a predetermined condition indicative of the presence of the fluid.

Methods and apparatus for assay measurements

A system having reduced gas interference that includes a fluid chamber and a resonant sensor device in fluid communication with a fluid in the fluid chamber. The system includes a fluid control device adapted to change at least one of the fluid flow or pressure within the fluid chamber to achieve substantial wetting of surfaces in proximity to the resonant sensor device. Fluid surfaces of the system can include a material to increase the wettability (e.g., hydrophilicity) of the fluid surfaces.

Resonant sensor systems and methods with reduced gas interference

The invention relates to a fluidic system that includes a body structure having a chamber disposed therein. The fluidic system includes at least one fluid input at a first end of the chamber and at least one fluid output at a second end of the chamber. The fluidic system also includes a sensor device (e.g., an acoustic device) having a surface defining a portion of a surface of the chamber. The fluidic system also includes a first surface at the first end of the chamber oriented at an oblique or arcuate (e.g., curved) angle relative to the surface of the sensor device to direct fluid through the chamber.

Fluidic system with improved flow characteristics

The technology provided herein generally relates to reusable detection surfaces and methods for reusing a detection surface after using the detection surface in an assay for an analyte.

Reusable detection surfaces and methods of using same

Methods and apparatuses for performing assays involving binding material elements with a plurality of bonds over a substantial area of a surface of a resonant device establishing a normalized exposure. The methods and apparatuses also involve controlling an external influence applied to the material elements over a first period of time and measuring a signal during a second period of time that is indicative of the change in the amount of material elements bound to the surface relative to the normalized exposure. In some cases, the measured signals are integrated with respect to time to determine the time averaged amount of material elements bound to the surface.

Microfabricated devices for operation in a fluid that include a substrate that has a first and second surface and a first electrode material layer located over the first surface of the substrate. The devices have a piezoelectric material layer located over the first electrode material layer and a second electrode material layer located over the piezoelectric material layer. The devices also include a layer of isolation material located over the second electrode material layer that at least one of chemically or electrically isolates a portion of the second electrode material layer from a fluid. Some devices include a layer of conductive material located over the layer of isolation material.

Microfabricated devices and method for fabricating microfabricated devices

The invention relates to micro-fabricated devices having a suspended membrane or plate structure and micro-fabrication techniques for making such devices. A substrate defines a cavity passing through the substrate, and the cavity defines a first opening. An intermediate portion is disposed over the substrate and defines a second opening. The second opening is larger in size than the first opening, and the dimensions of the second opening are controlled according to a parameter associated with performance of the device. A membrane is positioned adjacent the second opening.

Micro-fabricated devices having a suspended membrane or plate structure

Methods for detecting analytes in a sample are provided. A plurality of particles, each of which is coated with a capture agent having an affinity for the analyte, is combined with the sample to form a plurality of analyte-particle complexes. The system also includes a transport arrangement for transporting the sample to the sensor surface, and a magnetic field inducing structure constructed and arranged to establish a magnetic field at and adjacent to the sensor surface. The resonant sensor produces a signal corresponding to an amount of analyte-particle complexes that are bound to the sensor surface.

Method and apparatus for detection of analyte using an acoustic device

Methods for detecting viruses are provided. A plurality of particles, each of which is coated with a capture agent having an affinity for the virus, is combined with the sample to form a plurality of analyte-particle complexes. The system also includes a transport arrangement for transporting the sample to the sensor surface, and optionally a magnetic field inducing structure constructed and arranged to establish a magnetic field at and adjacent to the sensor surface. The resonant sensor produces a signal corresponding to an amount of analyte-particle complexes that are bound to the sensor surface.

Methods and apparatus for detecting viruses using an acoustic device

Methods for detecting bacteria are provided. A plurality of particles, each of which is coated with a capture agent having an affinity for the bacteria, is combined with the sample to form a plurality of analyte-particle complexes. The system also includes a transport arrangement for transporting the sample to the sensor surface, and optionally a magnetic field inducing structure constructed and arranged to establish a magnetic field at and adjacent to the sensor surface. The resonant sensor produces a signal corresponding to an amount of analyte-particle complexes that are bound to the sensor surface.

Methods and apparatus for detecting bacteria using an acoustic device

Methods for detecting estradiol and metabolites thereof in a sample are provided. A plurality of particles, each of which is coated with a capture agent having an affinity for estradiol, is combined with the sample to form a plurality of estradiol-particle complexes. The system also includes a transport arrangement for transporting the sample and/or particles to the sensor surface, and optionally a magnetic field inducing structure constructed and arranged to establish a magnetic field at and adjacent to the sensor surface. The resonant sensor produces a signal corresponding to an amount of estradiol-particle complexes that are bound to the sensor surface.

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Hollis, NH, United States of America

Michael Francis Miller