Innovaprep, LLC

Midwest Research Institute

Michael F Fischer

Andrew Edward Page

David Scott Alburty

Zachary Allen Packingham

Alec Douglas Adolphson

John D Birkenholz

Ann K Packingham

Steven Dale Graham

Brian R Cage

Alexander J Waller

Carroll E Ball

Joseph Matthew Langle

Paul G Gorman

Joseph W Clement

Darren J Radke

James M Egan

David H Steele

Alec D Adolphson

Zachary A Packingham

David S Alburty

Andrew E Page

Highly efficient and rapid filtration-based concentration devices, systems and methods are disclosed with sample fluidic lines and a filter packaged in a disposable tip which concentrates biological particles that are suspended in liquid from a dilute feed suspension. A sample concentrate or retentate suspension is retained while eliminating the separated fluid in a separate flow stream. The concentrate is then dispensed from the disposable tip in a set volume of elution fluid. Suspended biological particles include such materials as proteins/toxins, viruses, DNA, and/or bacteria in the size range of approximately 0.001 micron to 20 microns diameter. Concentration of these particles is advantageous for detection of target particles in a dilute suspension, because concentrating them into a small volume makes them easier to detect and identify. A single-use pipette tip includes fluid ports for aspirating the sample and connecting to a concentrating unit.

Liquid to liquid biological particle concentrator with disposable fluid path

Devices, systems and methods are disclosed which relate to using wet foam elution for removal of particles from swabs and wipes. This allow users to capture particles from surfaces and recover them by elution into small sample volumes for subsequent detection for human clinical, veterinary, food safety, pharmaceutical, outbreak investigations, forensics, biodefense and bioterrorism response, environmental monitoring, and other applications where collection of samples from surfaces and humans or animals is required. More specifically, the swabs or wipes are used to collect samples in the standard ways that commercially available swabs and wipes are in use today; from, for instance, food preparation surfaces in food plants, from production equipment in pharmaceutical facilities, for collection of dry powders during bioterrorism event response, and for collection of clinical samples such as nasal, throat, nasopharyngeal, and wounds.

Devices, systems and methods for surface sampling

A reel-to-reel aerosol collection and storage system for capturing and storing a plurality of separate samples of aerosol particles using electrostatic precipitation technology for collecting such particles onto a tape mechanism for analysis at a future time. The particles are collected onto a region of the collection tape for a defined period of time and then that portion of the collection tape is advanced out of the collection zone and manipulated so as to form sealed pouches or pockets which can be either severed from the collection tape for individual storage in a temperature controlled storage container, or which can be stored on a take-up reel in a temperature controlled environment. The collected aerosol particles are stored with a buffer fluid to preserve and maintain the viability of the collected particles for future extraction and analysis. Several embodiments for forming sealed pouches or pockets and methods of storing the collected particles are disclosed.

Reel-to-reel bioforensic aerosol collection and storage system

Dissolved components of a liquid sample are recovered for analysis by delivering the sample to a concentrator comprising a cylindrical receptacle into which an uncontaminated gas, such as air, is introduced in a direction tangentially of the cylindrical wall. The gas is under sufficient pressure to effect swirling of the gas and liquid sample and atomization of the sample to rapidly evaporate the same and cause a residue containing the dissolved components to be deposited on the interior surface of the wall. A reconstituting solvent is then admitted to the receptacle to dissolve the residue and provide a reconstituted, concentrated sample for analysis. If desired, the sample may be concentrated to a given volume rather than to complete dryness. In nonlaboratory applications, a dilute liquid substance may be processed by rapid evaporation to provide a final product of desired concentration.

Rapid evaporation method for analysis

Growing community of inventors

Lee's Summit, MO, United States of America

Michael F Fischer