Agriculture Victoria Services Pty Limited

Agresearch Limited

Michael Emmerling

German Carlos Spangenberg

Timothy Ivor Sawbridge

Stephen Panter

Eng-Kok Ong

Eng Kok Ong

Ramiro Mendez

Amanda Winkworth

Marcel Labandera

Jason Simmonds

The present invention relates to nucleic acid fragments encoding amino acid sequences for flavonoid biosynthetic enzymes in plants, and the use thereof for the modification of, for example, flavonoid biosynthesis in plants, and more specifically the modification of the content of condensed tannins. In particularly preferred embodiments, the invention relates to the combinatorial expression of chalcone synthase (CHS) and/or dihydroflavonol 4-reductase (BAN) and/or leucoanthocyanidine reductase (LAR) in plants to modify, for example, flavonoid biosynthesis or more specifically the content of condensed tannins.

Chalcone synthase dihyrdoflavonol 4-reductase and leucoanthocyanidine reductase from clover, medic ryegrass or fescue

The present invention relates to nucleic acids and nucleic acid fragments encoding amino acid sequences for flavonoid biosynthetic enzymes in plants, and the use thereof for the modification of flavonoid biosynthesis in plants. More particularly, the flavonoid biosynthetic enzyme is selected from the group consisting of chalcone isomerase (CHI), chalcone synthase (CHS), chalcone reductase (CHR), dihydroflavonol 4-reductase (DFR), leucoanthocyanidin reductase (LCR), flavonoid 3',5&#x2032; hydrolase (F3&#x2032;5&#x2032;H), flavanone 3-hydroxylase (F3H), flavonoid 3&#x2032;-hydroxylase (F3&#x2032;H), phenylalanine ammonia-olyase (PAL) and vestitone reductase (VR), and functionally active fragments and variants thereof.

Manipulation of flavonoid biosynthesis in plants

The present invention relates to nucleic acid fragments encoding amino acid sequences for organic acid biosynthetic enzymes in plants, and the use thereof for the modification of, for example, organic acid biosynthesis and secretion in plants. In particularly preferred embodiments, the invention relates to the combinatorial expression of citrate synthase (CS) and/or malate dehydrogenase (MDH) and/or phosphoenolpyruvate carboxylase (PEPC) in plants to modify, for example, organic acid synthesis and secretion.

Manipulation of organic acid biosynthesis and secretion

Chalcone synthase dihydroflavonol 4-reductase and leucoanthocyanidine reductase from clover, medic ryegrass or fescue

The present invention relates to polynucleotides encoding cinnamyl alcohol dehydrogenase (1) like (CAD1L) polypeptides. CAD1L polypeptides are produced in a plant in the same organs and the same developmental stages and processes of CAD1 and are likely to be involved in the same developmental processes as CAD enzymes. CAD1L-like sequences are also disclosed. They can be used for modification of, for example, lignification, cellulose, degradation, plant cell walls or plant defense response.

Plant CAD1-like genes and their use

The present invention relates to nucleic acids and nucleic acid fragments encoding amino acid sequences for flavonoid biosynthetic enzymes in plants, and the use thereof for the modification of flavonoid biosynthesis in plants. More particularly, the flavonoid biosynthetic enzyme is selected from the group consisting of chalcone isomerase (CHI), chalcone synthase (CHS), chalcone reductase (CHR), dihydroflavonol 4-reductase (DFR), leucoanthocyanidin reductase (LCR), flavonoid 3', 5&#x2032; hydrolase (F3&#x2032;5&#x2032;H), flavanone 3-hydroxylase (F3H), flavonoid 3&#x2032;-hydroxylase (F3&#x2032;H), phenylalanine ammonia-olyase (PAL) and vestitone reductase (VR), and functionally active fragments and variants thereof.

Growing community of inventors

Greensborough, Australia

Michael Emmerling