The State University of New York

Becton Dickinson and Company Limited

Michael E Perlman

Thomas J Bardos

Joan E MacDiarmid

Susan A Evans

A method of inhibiting the replication of tumor cells is disclosed wherein Novel bis (2,2-dimethyl-1-aziridinyl)phosphinic amide antineoplastic agents of the formula: ##STR1## wherein X is R and R' are each, independently, hydrogen, alkyl, substituted alkyl, phenyl and substituted phenyl, Y is alkyl or substituted alkyl of 1-10 carbon atoms and, Z is oxygen or sulfur is administered to tumor cells.

Novel bis(2,2-dimethyl-1-aziridinyl) phosphinic amides for use in the

A method for immunoassay for a ligand suspected to be present in a fluid includes use of an enzyme, a metal ion catalyst for an indicator reaction and a blocked modulator for the catalyst. Ligand present in the fluid binds to an antiligand. The resulting bound fraction activates the enzyme to unblock the modulator. The free modulator activates or inhibits the catalyst thereby modulating the rate of an indicator reaction between a substrate and a redox reagent. The presence of absence of the ligand in the fluid is indicated by a signal, such as a color change or a rate of color change, consequent to the indicator reaction. The invention includes a kit of materials useful for performing the method of the invention.

Signal enhancement in immunoassay by modulation of chemical catalysis

Novel phosphoaziridines of the formula: ##STR1## wherein Z is oxygen or sulfur, R.sub.1 is independently at each occurrence H or --CH.sub.3, R.sub.2 is independently at each occurrence --CH.sub.3 or nitrophenyl R.sub.5 ; R.sub.3 is --NHR.sub.4, --OR.sub.4, --SR.sub.4 or ##STR2## where R.sub.4 is a radical containing up to 30 atoms including at least one amine, ester, ether, alkyl, substituted alkyl, hydrogen, phenyl, substituted phenyl, carbamate, heterocyclic ring or R.sub.5 group; provided that, when R.sub.4 contains and R.sub.5 group, R.sub.4 has an alkyl group between the phosphorous and the R.sub.5 group; and R.sub.5 is a radical containing and electron affinic group comprising a quinone, or a heterocyclic or phenyl ring directly connected to a radical selected from the group consisting of nitro, nitroso, sulfinyl, sulfoxine, and N-oxide; provided that the compound contains at least one R.sub.5 radical and provided that when R.sub.1 is H, the R.sub.2 group on the same ring is R.sub.5 ; and the method for using the above compounds for the treatment of tumors.

Phosphoaziridine compounds useful for treatment of tumors

Novel bis(2,2-dimethyl-1-aziridinyl)phosphinic amide antineoplastic agents are disclosed of the formula: ##STR1## wherein R and R' are each, independently, hydrogen, alkyl, substituted alkyl, phenyl and substituted phenyl and, Z is oxygen or sulfur.

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Durham, NC, United States of America

Michael E Perlman