Gen9, Inc.

Evonik Corporation

Ginkgo Bioworks, Inc.

Michael E Hudson

Joseph M Jacobson

Daniel Schindler

Li-yun A Kung

Martin J Goldberg

Ishtiaq E Saaem

Andrew Wilson

Larry Li-Yang Chu

Senthil Ramu

Scott S Lawton

Li-Yun A Kung

Stephen F Archer

Sophea Sann-Rorth

Ellen J Sanchez

Stephen Archer

Ishtiaq Saaem

Devin Leake

Methods and apparatus of some aspects of the invention relate to the synthesis of high fidelity polynucleotides. In particular, aspects of the invention relate to concurrent enzymatic removal of amplification sequences and ligation of processed oligonucleotides into nucleic acid assemblies. According to some embodiments, the invention provides a method for producing a target nucleic acid having a predefined sequence. In some embodiments, the method comprises the step of providing a plurality of oligonucleotides, wherein each oligonucleotides comprises (i) an internal sequence identical to a different portion of a sequence of a target nucleic acid, (ii) a 5' sequence flanking the 5′ end of the internal sequence and a 3′ flanking sequence flanking the 3′ end of the internal sequence, each of the flanking sequence comprising a primer recognition site for a primer pair and a restriction enzyme recognition site.

Methods for nucleic acid assembly and high throughput sequencing

Methods and apparatus relate to the synthesis of polynucleotides having a predefined sequence on a support. Assembly methods include primer extension to generate overlapping construction oligonucleotides and assembly of the polynucleotides of interest onto an anchor support-bound oligonucleotides. Methods and apparatus for selection of polynucleotides having the predefined sequence and/or length are disclosed.

Methods and devices for nucleic acids synthesis

Aspects of the invention relate to methods, compositions for designing and producing a target nucleic acid. In particular, aspects of the invention relate to the multiplex synthesis of target polynucleotides.

Compositions and methods for multiplex nucleic acids synthesis

Methods and apparatus of some aspects of the invention relate to the synthesis of high fidelity polynucleotides. In particular, aspects of the invention relate to concurrent enzymatic removal of amplification sequences and ligation of processed oligonucleotides into nucleic acid assemblies. According to some embodiments, the invention provides a method for producing a target nucleic acid having a predefined sequence. In some embodiments, the method comprises the step of providing a plurality of oligonucleotides, wherein each oligonucleotides comprises (i) an internal sequence identical to a different portion of a sequence of a target nucleic acid, (ii) a 5' sequence flanking the 5&#x2032; end of the internal sequence and a 3&#x2032; flanking sequence flanking the 3&#x2032; end of the internal sequence, each of the flanking sequence comprising a primer recognition site for a primer pair and a restriction enzyme recognition site.

Methods and devices are provided for preparing a protein array having a plurality of proteins. In one embodiment, the method includes providing a plurality of nucleic acids each having a predefined sequence and expressing in vitro a plurality of proteins from the plurality of nucleic acids. In another embodiment, protein arrays having a solid surface and a microvolume are also provided. The solid surface can have a plurality of anchor oligonucleotides capable of hybridizing with a plurality of nucleic acids. The microvolume can cover each of the plurality of anchor oligonucleotides and can be configured to produce a polypeptide from each of the plurality of nucleic acids.

Protein arrays and methods of using and making the same

Methods and compositions relate to the sorting and cloning of high fidelity nucleic acids using high throughput sequencing. Specifically, nucleic acid molecules having the desired predetermined sequence can be sorted from a pool comprising a plurality of nucleic acids having correct and incorrect sequences.

Methods for sorting nucleic acids and multiplexed preparative in vitro cloning

Disclosed are methods, compositions and devices for screening a protein library for proteins having a desired activity, such as capable of catalyzing the formation of a bond between two reactants. In an exemplary embodiments, a plurality of proteins are expressed in vitro from a plurality of nucleic acids, the plurality of proteins are exposed with two single stranded oligonucleotides having complementary sequences, each oligonucleotide having a reactant and a fluorophore, the fluorescence of the protein-reactant-oligonucleotide-fluorophore complexes is detected and the complexes showing detectable fluorescence energy transfer are isolated, thereby isolating proteins having the desired enzymatic activity.

Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis

Aspects of the invention relate to methods, compositions for synthesizing high fidelity oligonucleotides.

Compositions and methods for synthesis of high fidelity oligonucleotides

The core-sheath implant device allows for a bioactive agent release profile that can be tailored to a particular therapy. The device features one or more lobes extending from an inner core at least partially through the outer sheath. Depending on the particular design of such an implant device, a variety of release profiles can be achieved.

Growing community of inventors

Framingham, MA, United States of America

Michael E Hudson