Apdn (b.v.1.) 1nc.

Apdn, Inc.

Michael E Hogan

Lawrence Jung

Frederick H Eggers

Yuhua Sun

Fushi Wen

Stephen Hughes

Melissa R May

Kevin M O'brien

Provided herein is a method for identifying a mastitis-causing microbe in a subject. A milk sample is centrifuged to form a microbial pellet, total nucleic acids are extracted from the pellet and a microarray analysis of extracted DNA from which the mastitis-causing microbe is identified from DNA hybridization to mastitis-causing microbe species-specific gene probes. Also provided is a method for diagnosing a bovine mastitis infection in a dairy cow after identifying the bovine mastitis-causing microbe in a raw milk sample from the dairy cow.

Method for identifying mastitis-causing microbes

The present invention relates to systems and methods to produce recombinant adeno-associated virus (rAAV) utilizing one or more DNA constructs manufactured via polymerase chain reaction (PCR).

Methods and systems of PCR-based recombinant adeno-associated virus manufacture

Provided herein is a method for detecting the presence of a COVID-19 virus RNA or other pathogenic respiratory viruses, such as an influenza virus, or other RNA of interest in a sample. Nucleic acids are obtained from the sample and are used as a template in a combined isothermal reverse transcription, RNAse H and isothermal amplification reaction to generate single stranded RNA amplicons containing sequences complementary to fluorescent labeled detector probes. The single-stranded RNA amplicons hybridize to the detector probe and to hybridization probes with sequences complementary to a sequence determinant in the COVID-19 or other virus RNAs. The microarray is imaged to detect fluorescent signals thereby identifying the virus.

Transcription mediated amplification methods for RNA detection

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Stony Brook, NY, United States of America

Michael E Hogan