Alliance for Sustainable Energy, LLC

Midwest Research Institute

The United States of America As Represented by the United States

Calgene Corporation

Alliance for Sustainably Enegry, LLC

Michael E Himmel

William S Adney

Stephen Robert Decker

Todd B Vinzant

Shi-You Ding

John O Baker

Yat-Chen Chou

Melvin P Tucker, Iii

Jeffrey G Linger

Steven R Thomas

Gregg Tyler Beckham

Karel Grohmann

Mark R Nimlos

Qi Xu

Roman Brunecky

Peter N Ciesielski

Eric E Jarvis

Andrew Nolan Wilson

Arjun Singh

Ruoran Zhang

Larry Taylor, Ii

Rafael A Nieves

Venkataramanan Subramanian

Kara Podkaminer

Min Zhang

David M Stalker

Mary Ann Franden

John Aikens

Richard T Elander

Charles E Wyman

Eric P Knoshaug

Ali Mohagheghi

David J Schaaf

Yannick J Bomble

Christopher J Richard

Xiaowen Chen

Michael F Crowley

Tina Jeoh

Robert A Laymon

Shih-You Ding

Suzanne Lantz McCarter

Suzanne Mc Carter

Raphael Nieves

The present disclosure relates to a composition that includes a base layer having an outer surface and a first thickness and a material that includes a plurality of cellulose nanofibers in physical contact with the outer surface, where the composition has an average filtration efficiency of less than or equal to 90% for particles having a characteristic length between about 50 nm and about 100 μm and the composition is characterized by an average inhalation resistance of less than or equal to 35 mm HO as measured across the first thickness and the material.

Cellulose-containing filtration materials and methods of making the same

Disclosed herein are processes for ethanol production from a lignocellulosic feedstock. These processes provide DMR of lignocellulosic biomass comprising two-stage deacetylation followed by mechanical refining so as to increase fermentable sugar yield while reducing hydrolytic enzyme loading requirements.

Pretreatment of lignocellulose using two stage alkali and mechanical refining processes

Disclosed herein are a cellulose particle and a binder, where the cellulose particle has a cellulose core and a surface group, and the binder interacts with the surface group to form a composite.

Cellulose-based composites and methods of making the same

Non-naturally occurringstrains useful for the production of 2,3-butanediol are provided.

Engineered zymomonas for the production of 2,3-butanediol

Polypeptide scaffolds comprising enzymatic proteins are provided. The enzymatic polypeptide scaffolds comprise heterologous enzymes to form a heterologous metabolic pathway, and can be targeted to a substrate through a surface anchoring domain. The enzymatic polypeptide scaffolds leverage the high specificity and affinity protein/protein interaction between the cohesins and dockerins of microorganismal cellulosomes to form custom enzymatic arrays.

Enzyme scaffolds and methods of use

Disclosed herein are methods of making multifunctional microbial cellulases. The engineered multifunctional microbial cellulases disclosed herein exhibit improved activity over native cellulases.

Multifunctional microbial cellulases

Disclosed herein are chimeric Cel7A polypeptides useful for producing biofuels from lignocellulosic biomass.

Fungal cellobiohydrolases

Nucleic acid sequences encoding chimeric polypeptides that exhibit enhanced cellulase activities are disclosed herein. These nucleic acids may be expressed in hosts such as fungi, which in turn may be cultured to produce chimeric polypeptides. Also disclosed are chimeric polypeptides and their use in the degradation of cellulosic materials.

Enhanced processive cellulases

Disclosed herein are enzymes and combinations of the enzymes useful for the hydrolysis of cellulose and the conversion of biomass. Methods of degrading cellulose and biomass using enzymes and cocktails of enzymes are also disclosed.

Enzymes for improved biomass conversion

Nucleic acid molecules encoding chimeric cellulase polypeptides that exhibit improved cellulase activities are disclosed herein. The chimeric cellulase polypeptides encoded by these nucleic acids and methods to produce the cellulases are also described, along with methods of using chimeric cellulases for the conversion of cellulose to sugars such as glucose.

Chimeric enzymes with improved cellulase activities

Provided herein is an isolated Cel7A polypeptide comprising mutations in the catalytic domain of the polypeptide relative to the catalytic domain of a wild type Cel7A polypeptide, wherein the mutations reduce N-linked glycosylation of the isolated polypeptide relative to the wild type polypeptide. Also provided herein is an isolated Cel7A polypeptide comprising increased O-linked glycosylation of the linker domain relative to a linker domain of a wild type Cel7A polypeptide. The increased O-linked glycosylation is a result of the addition of and/or substitution of one or more serine and/or threonine residues to the linker domain relative to the linker domain of the wild type polypeptide. In some embodiments, the isolated Cel7A polypeptide comprising mutations in the catalytic domain of the polypeptide relative to the catalytic domain of a wild type Cel7A polypeptide further comprises increased O-linked glycosylation of the linker domain relative to a linker domain of a wild type Cel7A polypeptide. The mutations in the catalytic domain reduce N-linked glycosylation of the isolated polypeptide relative to the wild type polypeptide. The addition of and/or substitution of one or more serine and/or threonine residues to the linker domain relative to the linker domain of the wild type polypeptide increases O-linked glycosylation of the isolated polypeptide. Further provided are compositions comprising such polypeptides and nucleic acids encoding such polypeptides. Still further provided are methods for making such polypeptides.

Cellobiohydrolase I enzymes

Purified cellobiohydrolase I (glycosyl hydrolase family 7 (Cel7A)) enzymes fromdemonstrate a high level of specific performance in comparison to other Cel7 family member enzymes when formulated with purified EIcd endoglucanase fromand tested on pretreated corn stover. This result is true of the purified native enzyme, as well as recombinantly expressed enzyme, for example, that enzyme expressed in a non-nativehost. In a specific example, the specific performance of the formulation using purified recombinant Cel7A fromexpressed inis increased by more than 200% when compared to a formulation using purified Cel7A from

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The invention provides a thermal tolerant (thermostable) cellulase, AviIII, that is a member of the glycoside hydrolase (GH) family. AviIII was isolated and characterized from, and, like many cellulases, the disclosed polypeptide and/or its derivatives may be useful for the conversion of biomass into biofuels and chemicals.

Growing community of inventors

Littleton, CO, United States of America

Michael E Himmel