Novozymes A/s

Other

Michael Dolbjerg Rasmussen

Ib Groth Clausen

Peter Bjarke Olsen

Steen Troels Jorgensen

Carsten Lillelund Olsen

Steen Troels Joergensen

Jens Tonne Andersen

Jens Toenne Andersen

Steen Troels J&oslash;rgensen

Martin Jon Persson

Amutant host cell derived from a parenthost cell, which mutant host cell is mutated in one or more gene(s) encoding one or more secreted polypeptide(s) which is at least 80% identical to one or more of the polypeptides shown in SEQ ID NO's: 2 to 248, wherein the mutant host cell secretes at least 5% less of the one or more secreted polypeptide(s) than the parent host cell, when they are cultivated under comparable conditions.

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The present invention solves the problem of integrating multiple copies of a gene of interest by homologous recombination into well defined positions adjacent to conditionally essential genes in a bacterial host strain chromosome, which already comprises at least one copy of the gene of interest in a different position.

Method for stable chromosomal multi-copy integration of genes

Growing community of inventors

Vallensbaek, Denmark

Michael Dolbjerg Rasmussen