Axcella Health Inc.

Lonza Sales Ag

Michael Doherty

Geoffrey Von Maltzahn

David Arthur Berry

Michael Hamill

Brett Adam Boghigian

Phillip Samayoa

Nathaniel Silver

Shaila Rahman

John Kramarczyk

Raymond W Bourdeau

David Young

Subhayu Basu

Ying-Ja Chen

Kathryn Heard

Caitlyn Harvey

Aaron R Noyes

Vimal Vaidya

Jameson Rogers

Konstantin Konstantinov

Douglas E Williams

Kathryn E Golden

Michael P Mercaldi

Kimberly Ellis

Kayla Desanty

Methods of loading extracellular vesicles with payload molecules via homogenization are disclosed herein.

Loading of extracellular vesicles through imparting of mechanical shear

The present disclosure relates to multistep chromatographic methods for preparing extracellular vesicles (EVs). The methods were demonstrated to be effective in preparing highquality EVs in a large scale. The methods enable preparation of EVs for therapeutic and diagnostic applications, and isolation and/or sub-fractionation of EVs with desired properties for specific use.

Process for preparing extracellular vesicles

Nutritive polypeptides are provided herein. Also provided are various other embodiments including nucleic acids encoding the polypeptides, recombinant microorganisms that make the polypeptides, vectors for expressing the polypeptides, methods of making the polypeptides using recombinant microorganisms, compositions and formulations that comprise the polypeptides, and methods of using the polypeptides, compositions and formulations.

Nutritive polypeptides and formulations thereof, and methods of production and use thereof

Growing community of inventors

Somerville, MA, United States of America

Michael Doherty