Duke University

Cargill Inc.

Lynch, Michael

Copley, Shelley

Louie, Michael

Michael David Lynch

Zhixia Ye

Hans H Liao

Eileen Spindler

Michael Tai Man Louie

Wendy Kathleen Ribble

Travis Robert Wolter

Tanya Lipscomb

Christopher Patrick Mercogliano

Ashley Trahan

Charles Cooper

Daniel Rodriguez

Ahmet Bozdag

Ron Evans

Shelley Copley

Michael Louie

Matthew Lipscomb

Romel Menacho Melgar

Tanya E W Lipscomb

Brittany Prather

Brittany Robinson

Dave A Hogsett

Tanya Lipscomb-Warnecke

David A Hogsett

Jeovanna Rios

Shuai Li

Charles Bridwell Cooper

Ashley Delanie Trahan

Chrisopher Patrick Mercogliano

Eileen Colie Spindler

Tanya Lipscomp

Methods and microorganisms for improved malonyl-CoA flux and production of products having malonyl-CoA as a precursor. The methods comprise dynamically regulating, in a stationary phase of a method, a nitrogen regulatory protein. The methods may dynamically regulate more than one gene.

Compositions and methods for improved malonyl-CoA biosynthesis using 2-stage dynamic metabolic control

The present disclosure provides compositions and methods for rapid production of chemicals in genetically engineered microorganisms in a large scale. Also provided herein is a high-throughput metabolic engineering platform enabling the rapid optimization of microbial production strains. The platform, which bridges a gap between current in vivo and in vitro bio-production approaches, relies on dynamic minimization of the active metabolic network.

Compositions and methods for metabolic control of a biofermentation process with synthetic metabolic valves

Described are a genetically modified microorganism and corresponding methods and products. The genetically modified microorganism may include a first gene that encodes an acyl transferase and a second gene that encodes a peptide or protein. One or both of the first and second gene may be heterologous. The genetically modified microorganism may include a modified acyl-CoA biosynthetic pathway configured for one or more of: inducible biosynthesis of an acyl-CoA and over-accumulation of the acyl-CoA. The genetically modified microorganism may be effective upon fermentation to cause acylation of the peptide or protein by the acyl transferase using the acyl-CoA to provide a N-acylated peptide or protein product.

In vivo protein N-acylation

This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a fatty acid or fatty acid derived product, wherein the modified microorganism produces fatty acyl-CoA intermediates via a malonyl-CoA dependent but malonyl-ACP independent mechanism.

Microorganisms and methods for the production of fatty acids and fatty acid derived products

Compositions and methods for robust dynamic metabolic control of 3-hydroxypropionic acid production

Compositions and methods for robust dynamic metabolic control of mevalonate production

Compositions and methods for robust dynamic metabolic control of a biofermentation process

Compositions and methods for screening microorganisms for robust dynamic metabolic control

The present disclosure is related to genetically engineered microbial strains and related bioprocesses for the production of pyruvate and related products. Specifically, the use of dynamically controlled synthetic metabolic valves to reduce the activity of enzymes known to contribute to pyruvate synthesis, leads to increased pyruvate production in a two-stage process rather than a decrease in production.

Compositions and methods for the production of pyruvic acid and related products using dynamic metabolic control

Compositions and methods for robust dynamic metabolic control of alanine production

This invention relates to metabolically engineered microorganisms, such as bacterial and or fungal strains, and bioprocesses utilizing such strains. These strains enable the dynamic control of metabolic pathways, which can be used to optimize production. Dynamic control over metabolism is accomplished via a combination of methodologies including but not limited to transcriptional silencing and controlled enzyme proteolysis. These microbial strains are utilized in a multi-stage bioprocess encompassing at least two stages, the first stage in which microorganisms are grown and metabolism can be optimized for microbial growth and at least one other stage in which growth can be slowed or stopped, and dynamic changes can be made to metabolism to improve the production of desired product, such as a chemical or fuel.

Compositions and methods for rapid and dynamic flux control using synthetic metabolic valves

The present invention provides various combinations of genetic modifications to a transformed host cell that provide increase conversion of carbon to a chemical product. The present invention also provides methods of fermentation and methods of making various chemical products.

Acetyl-CoA carboxylases

Growing community of inventors

Durham, NC, United States of America

Michael David Lynch