Li-cor, Inc.

Purdue Research Foundation

Michael D Furtaw

Donald Thomas Lamb

Dayle K McDermitt

Robert D Eckles

Jonathan M Welles

Georgiy G Burba

Lyle R Middendorf

Liukang Xu

Mark A Johnson

Daniel J Anderson

Jon P Anderson

Mary Jean Wirth

Yimin Hua

Zhaorui Zhang

Robert E Birdsall

Dayle K Mcdermitt

Devices, systems, and methods of using them are disclosed that position an end of a capillary electrophoresis tube within an internal tapered nozzle region of an inkjet print head or other microfluidic pump. The capillary electrophoresis tube can extend through an inlet of the microfluidic pump and leave space for a sheath liquid to enter the pump and mix with separated analytes eluted from the capillary electrophoresis tube. The small volume of mixed sheath liquid and analyte can then be jetted through the nozzle at a moving surface, either continuously or as discrete droplets. Relative positions on the surface can indicate separation distances of dispensed analytes.

Capillary electrophoresis inkjet dispensing

Devices and methods are provided for the separation and dispensing of material using a microfluidic electrophoresis column, sheath liquid pump, and exit channel, all on the same monolithic chip. Material is separated in the electrophoresis column and passed into the exit chamber in response to a voltage potential between a first electrode within the electrophoresis column and a terminating electrode integrated into the chip. The terminating electrode can be in the sheath liquid pump chamber, the sheath liquid reservoir, or a separate flow channel that intersects the exit channel along with the electrophoresis column and sheath liquid pump chamber. The flow of sheath liquid into the exit chamber entrains separated analytes into an effluent that is dispensed out of the exit chamber via a discharge outlet.

Microchip electrophoresis inkjet dispensing

Devices and methods are provided for the separation and dispensing of material using a microfluidic separation column connected via an exit channel to one or more sheath flow channels. The flow of separated material through the separation column is at least partially driven by a voltage potential between a first electrode within the separation column and a terminating electrode within at least one of the sheath flow channels. The separation column, exit channel, sheath flow channels, and electrodes are all within a single monolithic chip. The presence of an on-chip terminating electrode allows for separated material to be entrained in the sheath fluids and ejected onto a surface that can be non-conductive. The presence of multiple sheath flows allows for sheath flow fluids to have different compositions from one another, while reducing the occurrence of sheath flow fluids entering the separation column.

Methods for using multi-sheath flow and on-chip terminating electrode for microfluidic direct-blotting

Multi-sheath flow and on-chip terminating electrode for microfluidic direct-blotting

Devices, systems, and methods are disclosed that analyze a biological or other fluid sample using an electrophoresis or other separation method and then emit the fluid sample with separated constituents using an electrohydrodynamic spray to form a Taylor cone and jet, without dispersion into droplets, onto a substrate that moves with respect to the emitter. Electrodes can be shared between the electrophoresis and electrospray elements, and an adjunct fluid can help draw the separated sample into the Taylor cone. A micro-machined capillary channel on a chip can supply multiple lines to a substrate.

Analyte separator with electrohydrodynamic Taylor cone jet blotter

A self-assembled engineered lattice of nanometer-scale silica particles, or other suitable particles generally resembling regularly-sized spheres, is configured in a separation bed for electrophoresis, isoelectric focusing, chromatography, or other voltage-induced separation of analytes. After separation, the analytes are immobilized on the separation bed and then ionized using matrix-assisted laser desorption/ionization (MALDI) for use with a mass spectrometer. The nanoparticles can be coated with polymers that activate to immobilize the analytes or assist with MALDI. The separation can occur in two dimensions.

Laser desorption ionization mass spectrometry using a particulate separation bed

Gas analyzer systems and methods for measuring concentrations of gases and in particular dry mole fraction of components of a gas. The systems and methods allow for rapid measurement of the gas density and/or dry mole fraction of gases for a number of environmental monitoring applications, including high speed flux measurements. A novel coupling design allows for tool-free removal of a cell enclosing a flow path to enable infield cleaning of optical components and to enable re-configuration between open- and closed-path analyzer configurations. In closed path configurations, the sample flow path is thermally isolated from the remainder of the gas analyzer to provide more accurate temperature measurements. Composite plastic/metal sample cells are presented with achieve this thermal isolation without compromising analyzer performance.

Hybrid gas analyzer with thermally insulated flow cell

Gas analyzer systems and methods for measuring concentrations of gasses and in particular dry mole fraction of components of a gas. The systems and method allow for rapid measurement of the gas density and/or dry mole fraction of gases for a number of environmental monitoring applications, including high speed flux measurements. A novel coupling design allows for tool-free removal of a cell enclosing a flow path to enable in field cleaning of optical components.

Gas analyzer

Gas analyzer systems and methods for determining gas flux in a short intake tube configuration without using any Webb-Pearman-Leuning density correction. Gas analyzer systems and methods for measuring concentrations of gasses and in particular dry mole fraction of components of a gas. The systems and method allow for rapid measurement of the gas density and/or dry mole fraction of gases for a number of environmental monitoring applications, including high speed flux measurements. A novel coupling design allows for tool-free removal of a cell enclosing a flow path to enable in field cleaning of optical components.

Gas analyzer systems and methods for measuring concentrations of gasses and in particular dry mole fraction of components of a gas. The systems and method allow for rapid measurement of the gas density and/or dry mole fraction of gases for a number of environmental monitoring applications, including high speed flux measurements. A novel coupling design allows for tool-free removal of a cell enclosing a flow path to enable in field cleaning of optical components and to enable re-configuration between open- and closed-path analyzer configurations.

Hybrid gas analyzer

A method and apparatus are provided for maintaining pressure inside a closed chamber equal to the changing outside pressure, detecting leaks in the plumbing (i.e., air handling system) of a trace gas flux measurement system, and estimating the kinetic volume, e.g., of such a system.

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Lincoln, NE, United States of America

Michael D Furtaw