Louisiana State University and Agricultural & Mechanical College

Cornell University

The University of North Carolina at Chapel Hill

Northeastern University

Other

Michael C Murphy

Steven A Soper

Francis Barany

Robin L McCarley

Collin J McKinney

Sunggook Park

Mateusz Hupert

Dimitris Nikitopoulos

George Grills

John William Efcavitch

André A Adams

Elizabeth J Podlaha-Murphy

Daniel Sang-won Park

Dorel Moldovan

Patrick J Murphy

Jianmin Huang

Matthew A Johnson

Hong Wang

Malgorzata A Witek

Taehyun Park

Sudheer D Rani

June Feng

June Feng Schneider

Yu-wei Cheng

Andre A Adams

Dimitris E Nikitopoulos

Robin L Mccarley

Collin J Mckinney

Juan Feng

The present invention relates to a device comprising a biomolecular processor. Each biomolecular processor has one or more bioreactor chambers defined by a solid substrate; a support structure within each bioreactor; a cleaving enzyme immobilized to the support structure and operatively positioned within the bioreactor chamber to cleave monomer or multimer units of a biopolymer molecule operatively engaged by the cleaving enzyme; and one or more time-of-flight channels formed in the solid substrate and fluidically coupled to said one or more bioreactor chambers. Each of the time-of-flight channels have two or more sensors including at least (i) a first sensor contacting the time-of-flight channel proximate to the input end of the channel and (ii) a second sensor contacting the time-of-flight channel proximate to the output end of channel. The present invention further relates to methods of sequencing and identifying biopolymer molecules using the device.

Biomolecular processing platform and uses thereof

The present invention is directed to methods comprising a device that comprises a biomolecular processor and one or more nanotubes. Each biomolecular processor comprises a bioreactor chamber defined by a solid substrate, a plurality of spaced support structures within said bioreactor chamber and attached to the solid substrate, one or more nanotubes defined by the solid substrate and fluidically coupled to the bioreactor chamber and one or more capture molecules immobilized to some or all of said plurality of spaced support structures, said one or more capture molecules suitable to bind to a portion of a target nucleic acid molecule in a sample. The nanotubes have a passage extending between an input end proximate to the bioreactor chamber and an output end distal to the bioreactor chamber, and comprises one or more nanopores within the passage with each nanopore having a reduced diameter relative to the passage.

Universal molecular processor for precision medicine

Microdevices are disclosed to efficiently, accurately, and rapidly isolate and enumerate rare cells, such as circulating tumor cells, from liquids such as whole blood. The system employs multiple parallel meandering channels having a width on the order of 1-2 cell diameters. The microdevices can be produced at low-cost, may readily be automated, and in many instances may be used without pre-processing of the sample. They may be used to isolate and enumerate rare cells, including for example the detection and diagnosis of cancers, cancer staging, or evaluating the effectiveness of a therapeutic intervention, or detecting pathogenic bacteria. The device may optionally be used to nondestructively capture and later to release target cells.

Microfluidic isolation of tumor cells or other rare cells from whole blood or other liquids

The present invention is directed to a device that comprises a biomolecular processor and one or more nanotubes. Each biomolecular processor comprises a bioreactor chamber defined by a solid substrate, a plurality of spaced support structures within said bioreactor chamber and attached to the solid substrate, and one or more capture molecules immobilized to some or all of said plurality of spaced support structures, said one or more capture molecules suitable to bind to a portion of a target nucleic acid molecule in a sample. The device also comprises one or more nanotubes defined by the solid substrate and fluidically coupled to the bioreactor chamber.

The present invention is directed to methods for capturing, amplifying and identifying one or more of a plurality of target nucleotide sequences in a sample. The present invention is further directed to a device comprising a solid support having a plurality of wells or pillars and a plurality of oligonucleotides attached to the wells or pillars. Other aspects of the invention are directed to methods of making such devices.

Methods and devices for DNA sequencing and molecular diagnostics

A device and methods are provided for efficient and quick capture of target cells through a main microchannel having capture elements immobilized thereon and manipulating a velocity profile of a sample as it passes through the main microchannel. The cell capture device may have a main microchannel with a depth slightly larger than the diameter of the target cells and a plurality of side microchannels. The side microchannels may have a depth smaller than the diameter of the target cells. The device and methods may be used for early cancer detection.

Methods of and devices for capturing circulating tumor cells

An electronic vertical angle sensing and indicating device for use on aiming systems is provided for bow sights and for other aiming sights for projectile launchers. Improved vertical level measurement and display minimizes the left-right drift of a projectile by sensing and indicating to the user when the projectile launcher is tilted slightly prior to release of the projectile. The vertical level indicator is easily viewed within the field of direct or near direct vision and focus of the eye in a reduced profile that does not distract the operator from the task of accurately aiming the projectile is provided. One embodiment of the present invention positions the signal indicators in the far-field of view of the eye, so that all of the signal indicators, the sighting means and the distant target being viewed are simultaneously in focus or near-focus.

Digital vertical level indicator for improving the aim of projectile launching devices

Growing community of inventors

Baton Rouge, LA, United States of America

Michael C Murphy