US Government As Represented by the Secretary of the Army

Michael C Hevey

Alan L Schmaljohn

Peter M Pushko

Jonathan F Smith

Diane L Negley

Diane I Negley

Case C Grogan

Chimeric GP molecules were constructed which contain portions of both the EBOV and MBGV GP proteins by swapping the subunits between EBOV and MBGV. The chimeric molecules were cloned into an alphavirus replicon which offers the advantage of high protein expression levels in mammalian cells and is a proven vaccine vector. These chimeric molecules fully protected guinea pigs from MBGV challenge, and conversely protected the animals from EBOV challenge. These results indicate that a protective epitope resides within the GP2 subunit of the MBGV GP protein and at least partially within the GP2 subunit of the EBOV GP protein. Additionally these results show that a construction of a single-component bivalent vaccine protective in guinea pigs is achievable.

Chimeric filovirus glycoprotein

Using the MBGV GP, NP, and virion proteins, a method and composition for use in inducing an immune response which is protective against infection with MBGV in nonhuman primates is described.

Methods for producing Marburg virus proteins

Venezuelan equine encephalitis virus replicon encoding marburg virus proteins

The invention here relates to recombinant DNA constructs which comprise a Venezuelan equine encephalitis replicon vector and at least one DNA fragment encoding a protective antigen from the Marburg virus. The DNA constructs are useful for inducing an immune response which is protective against infection with Marburg virus in nonhuman primates.

Marburg virus vaccines

Growing community of inventors

Frederick, MD, United States of America

Michael C Hevey