Nuclera Nucleics Ltd.

Nuclera Ltd

Michael C Chen

Gordon R McInroy

Jiahao Huang

Radu A Lazar

Gordon Mcinroy

The invention relates to methods of synthesizing polymers, biopolymers, and nucleic acids, to immobilised dNTP/NTPs and kits comprising said immobilised dNTP/NTPs for use in said methods of nucleic acid synthesis.

Polymer synthesis using electrowetting devices

The invention relates to the use of a modified terminal transferase enzyme in a method of adding one or more nucleotides to the 3' end of a nucleic acid. The invention also relates to methods of nucleic acid synthesis and sequencing comprising the use of said modified terminal transferase enzyme, to kits comprising said modified terminal transferase enzyme and to the use of said kits in methods of nucleic acid synthesis and sequencing.

Use of terminal transferase enzyme in nucleic acid synthesis

The invention relates to the use of specific terminal deoxynucleotidyl transferase (TdT) enzymes in a method of nucleic acid synthesis, to methods of synthesizing nucleic acids, and to the use of kits comprising said enzymes in a method of nucleic acid synthesis. The invention also relates to the use of terminal deoxynucleotidyl transferases and 3'-blocked nucleotide triphosphates in a method of template independent nucleic acid synthesis.

Compositions and methods related to nucleic acid synthesis

The invention relates to the use of polyphosphate containing species in a method of nucleic acid synthesis, to methods of nucleic acid synthesis, and to the use of kits comprising said polyphosphate containing species. The invention also relates to the use of polyphosphate containing species for the capping of 3'-terminal hydroxyl moieties using terminal transferases.

Methods of nucleic acid synthesis comprising polyphosphate containing species

The invention relates to the use of an amine masked moiety in a method of enzymatic nucleic acid synthesis. The invention also relates to said amine masked moieties per se and a process for preparing nucleotide triphosphates comprising said amine masked moieties.

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Cambridge, United Kingdom

Michael C Chen