Degussa Aktiengesellschaft

Forschungszentrum Julich Gmbh

Forschungszentrum Juelich Gmbh

Roche Diagnostics Gmbh

Michael Bott

Brigitte Bathe

Lothar Eggeling

Natalie Schischka

Stephan Binder

Georg Schendzielorz

Steffen Schaffer

Stephanie Bringer-Meyer

Solvej Siedler

Maike Kortmann

Meike Baumgart

Peter Dimroth

Karin Schneider

Julia Frunzke

Nurije Mustafi

Axel Niebisch

An NADP(H) nanosensor has i) a nucleic acid sequence to which a regulator is capable of binding, wherein the oxidation state of the regulator depends on the NADP(H) availability; ii) a promoter sequence following the nucleic acid sequence i), to which an RNA polymerase is capable of binding, wherein the affinity of the RNA polymerase for the promoter sequence is influenced by the oxidation state of the regulator; iii) a nucleic acid sequence which is under the control of the promoter sequence ii) and which codes for an autofluorescent protein. The present invention also relates to a cell, a method for isolating genes which code for NADP(H)-dependent enzymes, and the use of an NADP(H) nanosensor.

Sensor for NADP (H) and development of alcohol dehydrogenases

A DNA sequence that includes at least 70% identity with respect to SEQ ID NO: 1 and further includes a triplet at position 1027-1029 that codes for alanine.

Pyruvate carboxylase and pyruvate carboxylase-encoding DNA, plasmid containing said DNA and microorganism for the production thereof, and methods for the production of products the biosynthesis of which includes oxaloacetate as precursor, and chromosome

The present invention relates to an NADP(H) nanosensor comprising The present invention also relates to a cell, a method for isolating genes which code for NADP(H)-dependent enzymes, and the use of an NADP(H) nanosensor.

A method for producing vectors containing a gene coding for an enzyme having reduced or deactivated feedback inhibition and to the use thereof for producing amino acids and nucleotide. Genes coding for feedback-inhibited enzymes are mutagenized in vitro, &#x2014;ligated into vectors in a further step&#x2014;the vectors are each transformed in a microorganism containing a metabolite sensor in a further step, which brings about the synthesis of a fluorescent protein at an increased metabolite concentration, &#x2014;whereupon microorganisms exhibiting increased or maximum fluorescence are selected; and &#x2014;the vectors that contain a gene coding for an enzyme having reduced or deactivated feedback inhibition are isolated from microorganisms having increased or maximum fluorescence.

Method for producing vectors containing a gene coding for an enzyme having reduced or deactivated feedback inhibition and the use thereof for producing amino acids and nucleotides

The present invention provides nucleotide sequences from Coryneform bacteria which code for the MtrA and/or MtrB proteins and processes for the fermentative preparation of amino acids using bacteria in which the mtrA and/or mtrB genes are attenuated.

Nucleotide sequences coding for the MtrA and/or MtrB proteins

The present invention provides nucleotide sequences frombacteria which code for the MtrA and/or MtrB proteins and processes for the fermentative preparation of amino acids using bacteria in which the mtrA and/or mtrB genes are attenuated.

Process for the production of a protein with citrate lyase activity by expressing a suitable plasmid in a host organism and isolating the protein in an active form, wherein the plasmid contains the information from a gene cluster composed of at least six genes and an inducible promoter. Furthermore the invention concerns the use of the recombinant enzyme and a corresponding test kit for the determination of citric acid.

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Juelich, Germany

Michael Bott