Endo Operations Limited

Calcigen Corporation

Endo Global Aesthetics Limited

Michael Berbaum

John Hanna

Daniel Shanafelt

Christine A Sheaffer

Jason Dziadosz

The present invention relates to the fields of collagenase production and collagenase products, and particularly to improving the reproducibility, purity, and stability of collagenase I and collagenase II compositions, where the compositions are pure to at least 95% by area as measured by reverse phase high pressure liquid chromatography (RP-HPLC) and essentially free of neutral protease.

Method of purifying and isolating collagenase

Method of producing collagenase

Methods of making high molecular weight hyaluronates are disclosed. The methods include contacting a hyaluronate-containing source with an acid to make an acidic hyaluronate suspension, contacting that suspension with an anionic ion exchange medium in the presence of an acidic buffer, and thereafter contacting the medium with an acidic buffer having a higher salt content to desorb the hyaluronate from the medium. The methods are amenable for isolating hyaluronate from such sources as avian (e.g., chicken, duck, turkey, or goose) eggshells and connective tissues of animals.

Production of high molecular weight hyaluronates

Growing community of inventors

Pottstown, PA, United States of America

Michael Berbaum