Henkel Kommanditgesellschaft Auf Aktien

Henkel Kommanditgesellschaft Auf Aktien (henkel Kgaa)

Henkel Research Corporation

Michael Bahn

Rolf Schmid

Joachim Schindler

Wolfgang Preuss

Rudiger Wagner

Ludwig Schieferstein

Christian G Paech

Werner Stein

Charles R Wilson

Konrad Engelskirchen

Robert B Reynolds

Dieter Hansen

Sherman S Hom

Jonathan R Mielenz

Beth F Ladin

Martina Markgraf

Karlheinz Maurer

Nicholas C Kennedy

A proteolytic enzyme for use in detergent formulations having increased pH and oxidative stability under typical laundering conditions in aqueous solutions is produced by fermenting Bacillus licheniformis host strain transformed by a multicopy plasmid comprised of DNA sequences which code for the desired proteolytic enzyme.

Alkaline proteolytic enzyme and method of production

In the aerobic culture of microorganisms of the Xanthomonas genus in fermentation media, the use of water-in-oil emulsions in the media minimizes viscosity problems and enhances yields of Xanthomonas biopolymers. Preferably, the emulsion is formed with a surfactant and the microorganism is Xanthomonas campestris.

Process for producing Xanthomonas biopolymers

The degradation of steroid-C22-carboxylic acids to the C20-carbonyl steroids is carried out in simplified form and preferably without isolating intermediate reaction products by using 17(20)-steroid-22-carboxylic acids as starting material and directly transforming them into the 20-carbonyl compound through their respective acid halides by the Curtius degradation or by carboxy-inversion degradation. The acetyl substituent thus formed in C17 of the steroid compounds is present in the pharmacologically required configuration.

Simplified process for the degradation of steroid-C-22-carboxylic acids

A .DELTA..sup.1,4,17 -BNC compound having the formula: ##STR1## wherein X is a substituent selected from the group consisting of OH and OCH.sub.3 ; as well as its process of production by microbiological side-chain splitting of a 17-C-side-chain steroid with a defect mutant microorganism which gives a steroid with a 17-C-.alpha.-propionic acid substituent in the absence of inhibitors inhibiting either the cleavage of the steroid ring or microorganism growth, where the defect mutant microorganisms are grown under a reduced air supply whereby an enrichment of .DELTA..sup.1,4,17 -BNC occurs.

3-Oxo-pregna-1,14,17-trien-20-carboxylates and process

Growing community of inventors

Hilden, Germany

Michael Bahn