Monsanto Technology LLC

Ecogen, Inc.

Monsanto Company

Michael A Von Tersch

Thomas M Malvar

Leigh H English

James William Bryson

Susan M Brussock

Caroline A Kulesza

Frederick S Walters

Stephen L Slatin

Charles P Romano

Jose M Gonzalez

Disclosed are nucleic acid segments comprising synthetically-modified genes encoding Coleopteran-toxic&#x3b4;-endotoxins. Also disclosed are methods of using these genes for the recombinant expression of polypeptides, the preparation of vectors containing the genes, and methods for transforming suitable host cells.

Coleopteran-resistant transgenic plants and methods of their production

The invention provides transgenic plants and transformed host cells which express modified cry 3B genes with enhanced toxicity to Coleopteran insects. Also disclosed are methods of making and using these transgenic plants, methods of making recombinant host cells expressing these &#x3b4;-endotoxins, and methods of killing insects such as Colorado potato beetle (Leptinotarsa decemlineata), southern corn rootworm (Diabrotica undecimpunctata howardi Barber) and western corn rootworm (Diabrotica virgifera virgifera LeConte.

Insect-resistant transgenic plants

Disclosed are nucleic acid segments comprising synthetically-modified genes encoding Coleopteran-toxic &dgr;-endotoxins. Also disclosed are methods of using these genes for the recombinant expression of polypeptides, the preparation of vectors containing the genes, and methods for transforming suitable host cells.

Nucleic acid compositions encoding modified Bacillus thuringiensis coleopteran-toxic crystal proteins

Disclosed are nucleic acid segments comprising synthetically-modified genes encoding modified Coleopteran-toxic Cry 3Bb* &dgr;-endotoxins. Also disclosed are methods of using these genes for preparing a Coleopteran-resistant transgenic plant and reducing insect in restations, and plants thereby produced.

Coleopteran-resistant transgenic plants and methods of their production using modified Bacillus thuringiensis Cry3Bb nucleic acids

Disclosed are methods for increasing the activity of B. thuringiensis .delta.-endotoxins against Coleopteran insect pests. Also disclosed are methods for mutagenizing nucleic acid sequences encoding these polypeptides, and increasing insect resistance in transgenic plants expressing these genes.

Methods for improving the activity of .delta.-endotoxins against insect

Disclosed are Coleopteran-toxic B. thuringiensis .delta.-endotoxins, nucleic acid sequences, and transgenic plants expressing these genes. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells.

Polypeptide compositions toxic to coleopteran insects

Disclosed are nucleic acid segments comprising synthetically-modified genes encoding Coleopteran-toxic B. thuringiensis .delta.-endotoxins. Also disclosed are methods of using these genes for the recombinant expression of polypeptides, the preparation of vectors containing the genes, and methods for transforming suitable host cells.

Nucleic acid segments encoding modified bacillus thuringiensis

The invention provides transgenic plants and transformed host cells which express modified cry 3B genes with enhanced toxicity to Coleopteran insects. Also disclosed are methods of making and using these transgenic plants, methods of making recombinant host cells expressing these .delta.-endotoxins, and methods of killing insects such as Colorado potato beetle (Leptinotarsa decemlineata), southern corn rootworm (Diabrotica undecimpunctata howardi Barber) and western corn rootworm (Diabrotica virgifera virgifera LeConte.

A Bacillus thuringiensis strain isolate, designated EG5092, exhibits insecticidal activity against lepidopteran insects. A purified and isolated novel cryET1 toxin gene product from B.t. strain EG5092 exhibits specific insecticidal activity against Plutella xylostella (Diamondback moth). The cryET1 gene (SEQ ID NO:1) has a nucleotide base sequence illustrated in FIG. 1 and produces a CryET1 gene product (SEQ ID NO:2) having a deduced amino acid sequence illustrated in FIG. 1.

Growing community of inventors

Ewing Township, NJ, United States of America

Michael A Von Tersch